Morphology Amastigote(leishman-donovan body) Human phase reside in macrophage >Very, very minute elliptical body > No free flagellum >Nucleus: deep red, located at one side >Cytoplasm: blue(after right stain) Kinetoplast; basal body rhizoplast
Morphology ▪ Amastigote (leishman-donovan body): ➢Human phase, reside in macrophage ➢Very, very minute elliptical body ➢No free flagellum ➢Nucleus: deep red, located at one side ➢Cytoplasm: blue (after right stain) ➢Kinetoplast; basal body; rhizoplast
Promastigote elongate form anterior PROMASTIGOTE 15-30 um long Flagellum one nucleus, red stained, Basal bod +Kinetoplast central in position one anterior flagellum which is just as long Nucleus as its body length. a rod like kinetoplast posterior Insect a dot-like basal body Motile Midgut
Promastigote elongate form - 15 - 30 µm long one nucleus, red stained, central in position, • one anterior flagellum which is just as long as its body length. • a rod-like kinetoplast • a dot-like basal body Flagellum Basal body Kinetoplast Nucleus anterior posterior Insect Motile Midgut
a Promastigote >Vector phase Reside in the gut of sandfly Spindle shaped with 1 free flagellum Nucleus; cytoplasm; kinetoplast; basal body; rhizoplast Chrysanthemum-like in culture medium
▪ Promastigote: ➢Vector phase ➢Reside in the gut of sandfly ➢Spindle shaped with 1 free flagellum ➢Nucleus; cytoplasm; kinetoplast; basal body; rhizoplast ➢Chrysanthemum-like in culture medium
Leishmania A macrophage filled with Leishmania amastigotes (by Mike Belosevic
▪ A macrophage filled with Leishmania amastigotes
Amastigote of L donovani (under oil immersion objective) 0.C 8x8
Amastigote of L. donovani (under oil immersion objective)