TABLE 302-a Pesticide Analytical Manual Vol I Table 302-a: Recovery Through 302 (E1-E3 +DG1-DG19) Chemical Recover Detector, wide bore or DEGS column required tolylfluanid Ccc toxaphene tralkoxydim V(98-106%) Recoveries of two Ov-101 peaks are different from one another tralomethrin tri-allate triadimefon Wide bore column recommended triadimenol Wide bore column recommended trlazamate triazophos tribufos trichlorfon Often converts to dichlorvos on glc column Wide bore or degs column required tricyclazole n or s detector required; wide bore column recommended tridiphane tretamine Ccccccccc Recovery tested at 0. Il and 0.55 ppm. triflumizole Wide bore column recommended trifluralin triflusulfuron methyl ester V(67-106%) hosphate tris(2-ethylhexyl) phosphate C(68-112%) mean recovery 97.6%0, n=ll tris(beta-chloroethyl) phosphate tris(chloropropyl)phosphate CCc May break down in solution. Temp program separated from interference in tomato vamidothion sulfone inclozolin metabolite b Severely subject to influence of matrix; levels 0 Ppm ha inclozolin metabolite e Severely subject to influence of matrix; levels <1.0 Ppm had very high recovery inclozolin metabolite f R Poor chromatography, influence of matrix pre- inclozolin metabolite s V(59-137%) TABLE 302-a--16 Transmittal No 2000-1(10/1
TABLE 302-a Pesticide Analytical Manual Vol. I TABLE 302-a—16 Chemical Recovery1 Notes2 Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19) Transmittal No. 2000-1 (10/1999) Form FDA 2905a (6/92) THPI C N detector, wide bore or DEGS column required. tolylfluanid C toxaphene C tralkoxydim V (38-106%) Recoveries of two OV-101 peaks are different from one another. tralomethrin C tri-allate C triadimefon C Wide bore column recommended. triadimenol C Wide bore column recommended. triazamate C triazophos C tribufos C trichlorfon C Often converts to dichlorvos on GLC column. Wide bore or DEGS column required. tricyclazole C N or S detector required; wide bore column recommended. tridiphane C trietazine C Recovery tested at 0.11 and 0.55 ppm. triflumizole C Wide bore column recommended. trifluralin C triflusulfuron methyl ester V (67-106%) triphenyl phosphate C tris(2-ethylhexyl) phosphate C (68-112%) mean recovery 97.6%, n=11 tris(beta-chloroethyl) phosphate C tris(chloropropyl) phosphate C Tycor C May break down in solution. Temp program separated from interference in tomato. vamidothion sulfone C vinclozolin C vinclozolin metabolite B C Severely subject to influence of matrix; levels <1.0 ppm had very high recovery. vinclozolin metabolite E C Severely subject to influence of matrix; levels <1.0 ppm had very high recovery. vinclozolin metabolite F R Poor chromatography, influence of matrix prevent quantitation of recovery. vinclozolin metabolite S V (59-137%)
Pesticide Analytical Manual Vol I TABLE 302-b Table 302-b: Recovery of Chemicals Through Method 302 (E1-E3 +C5+DG1-DG19J acetone extraction, partitioning or Hydromatrix removal of water, Florisil column cleanup, GLC determination with various columns and detectors) Che Recovery Eluant. c5 ot 2.3-dihyd benzofuranol methyl sulfonate NR No elution from Florisil only in 15% EE/PE 2. 6-dichlorobenzamide 83%elution from Florisil only in 200 mL thyl ethe 2-hydroxy-2, 3-dihydro-3, 3-methyl benzofuranol methyl sulfonate NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. 2-methoxy-3, 5, 6-trichloropyridine P(65%) 15 Low temperature column recommended. acetochlor 15+50 80% eluted in 15%EE/PE. P(68%)15 15 allethrin C(80%) Eluted in 50%EE/PE. 15 BHC, alpha 15 BHC. beta- 15 BHC, delta- bifenthrin 15 C(83%)15 bioresmethrin Some elution from Florisil in 200 mL 50% EE/PE, more in 200 mL 75%EE/PE biphenyl FID required br 15 bromopropylate C(80%)50 captafol Some elution from Florisil in 50% EE/PE after 15 %0 Codes: C: complete(>80%); P: Partial (50-80%); S: small (<50%): V: variable(approximate percentage when known); R: recovered but no quantitative information available; NR: not recovered 2 Recovery results refer to complete method; blank entry in this column indicates Florisil elution was tested but not complete method Eluants(s)in which chemical is eluted from Florisil, according to directions in 302 C5, i.e., 15 and 50%o ethyl ether/petroleum ether (EE/PE). Entries for chemicals not recovered indicate which eluants were used in tests. a "Florisil only " refers to tests in which elution patterns were tested by added reference standard solutions directly to Florisil a.2o110/1999 TABLE 302-b-1
TABLE 302-b–1 Pesticide Analytical Manual Vol. I TABLE 302-b Transmittal No. 2000-1 (10/1999) Form FDA 2905a (6/92) 1 Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known); R: recovered but no quantitative information available; NR: not recovered. 2 Recovery results refer to complete method; blank entry in this column indicates Florisil elution was tested but not complete method. 3 Eluants(s) in which chemical is eluted from Florisil, according to directions in 302 C5, i.e., 15 and 50% ethyl ether/petroleum ether (EE/PE). Entries for chemicals not recovered indicate which eluants were used in tests. 4 “Florisil only” refers to tests in which elution patterns were tested by added reference standard solutions directly to Florisil column. Table 302-b: Recovery of Chemicals Through Method 302 (E1-E3 + C5 + DG1-DG19) (acetone extraction, partitioning or Hydromatrix removal of water, Florisil column cleanup, GLC determination with various columns and detectors) Chemical Recovery1,2 Eluant, C53 Notes4 2,3-dihydro-3,3-methyl-2-oxo= -5-benzofuranyl methyl sulfonate NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. 2,6-dichlorobenzamide NR 50 83% elution from Florisil only in 200 mL ethyl ether. 2-hydroxy-2,3-dihydro-3,3-methyl= -5-benzofuranyl methyl sulfonate NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. 2-methoxy-3,5,6-trichloropyridine P (65%) 15 Low temperature column recommended. acetochlor C 15+50 80% eluted in 15% EE/PE. alachlor P (68%) 15 aldrin C 15 allethrin C (80%) 15 atrazine C 15+50 Eluted in 50% EE/PE. azinphos-ethyl C 15 BHC, alpha- C 15 BHC, beta- C 15 BHC, delta- C 15 bifenthrin C 15 binapacryl C (83%) 15 bioresmethrin NR 15 Some elution from Florisil in 200 mL 50% EE/PE, more in 200 mL 75% EE/PE. biphenyl C 15 FID required. bromophos C 15 bromopropylate C (80%) 50 bupirimate S (10-30%) 15 captafol NR 15 Some elution from Florisil in 50% EE/PE after 15%
TABLE 302-b Pesticide Analytical Manual Vol I Table 302-b: Recovery Through 302 (E1-E3+ C5+DG1-DG19) Chemical 2 Eluant c5 Notes captan Additional elution from Florisil in 50% EE/ carfentrazone ethyl ester Some additional elution in 50% EE/PE chlorbenside C chlordane chlordane. cis- C chlordane. trans- chlordimeform hydrochloride chlorfenapyr 15+50 chlorflurecol methyl ester No elution from Florisil only in 15% EE/PE 0%o not tested chlornitrofen chlorothalonil C(81%)15 Additional elution from florisil in 200 mL 50% EE/PE chlorpyrifos chlorpyrifos oxygen analog NR 555 Elution from Florisil with 50% EE/PE not tested chlorpyrifos-methyl C hlorthiophos oxygen analog No elution from Florisil only in 15%EE/PE 50% not tested hlorthiophos sulfone S(8%) chlorthiophos sulfoxide No elution from Florisil only in 15%EE/PE; clofentezine S(27-50%)15 Complete elution from Florisil only; de- grades on GLC in presence of extract. 6-15-50 No elution from Florisil only in 6, 15, or 50% EE/PE or 100%EE 15 DDE, P, P C DDT,P,P deltamethrin 15 ry poor eC detector sensitiv Dichlofluanid C 15+50 60% eluted in 15 EE/PE TABlE 302-b-2 Trans N.2o1(1
TABLE 302-b Pesticide Analytical Manual Vol. I TABLE 302-b–2 Transmittal No. 2000-1 (10/1999) Form FDA 2905a (6/92) Chemical Recovery1,2 Eluant, C53 Notes4 Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19) captan S (25%) 15 Additional elution from Florisil in 50% EE/ PE after 15%. carfentrazone ethyl ester C 15 Some additional elution in 50% EE/PE possible. chlorbenside C 15 chlordane C 15 chlordane, cis- C 15 chlordane, trans- C 15 chlordimeform hydrochloride NR 15-50 chlorfenapyr C 15+50 chlorflurecol methyl ester NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. chlornitrofen C 15 chlorothalonil C (81%) 15 Additional elution from Florisil in 200 mL 50% EE/PE. chlorpyrifos C 15 chlorpyrifos oxygen analog NR 15 Elution from Florisil with 50% EE/PE not tested. chlorpyrifos-methyl C 15 chlorthiophos oxygen analog NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. chlorthiophos sulfone S (8%) 15 chlorthiophos sulfoxide NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. clofentezine S (27-50%) 15 Complete elution from Florisil only; degrades on GLC in presence of extract. cymiazole NR 6-15-50 No elution from Florisil only in 6, 15, or 50% EE/PE or 100% EE. cypermethrin C 15 DCPA C 15 DDE, o,p'- C 15 DDE, p,p'- C 15 DDT, o,p'- C 15 DDT, p,p'- C 15 deltamethrin C 15 Very poor EC detector sensitivity. diazinon C 15 dichlofluanid C 15+50 60% eluted in 15% EE/PE
Pesticide Analytical Manual Vol I TABLE 302-b Table 302-b: Recovery Through 302 (E1-E3 +C5+DG1-DG19) Recovery. Eluant, C5 Notes diclobutrazol 15 56-70% elution from Florisil only; 50%not Pcccc tested; wide bore column recommended dicloran 15 dicofol, O,P 15 15 May be variable dieldrin lifenoxuron No elution from Florisil only in 15% EE/PE 50% not tested dimethachlon 50% not tested. orisil only in 15% EE/PE 15 endosulfan li endosulfan sulfate 15 Additional elution possible in 50%EE/PE. 15 15 15 30% elution from Florisil only; 50% not tested wide bore column recommended fenfuram P(45%) 15 Partial recovery also when Florisil eluted with Meci2 eluant #3 thin 15 fensulfothion sulfone No elution from Florisil only in 50%EE/PE fenvalerate 15 flamprop-M-isopropy Complete elution from Florisil only in 50% EE/PE plus additional EE flamprop-methyl 6-15-50 No elution from Florisil only in 6, 15, or 50% EE/PE; complete elution with EE fluchloralin flucythrinate 15+50 About 80% eluted in 15 EE/PE flusilazole 15 35-44% elution from Florisil only; wide bore column recommended fluvalinate FOE 504% thioglycolate sulfoxide NR lot eluted from florisil 78% eluted in 15% EE/PE a.2o110/1999 TABLE 302-b-3
TABLE 302-b–3 Pesticide Analytical Manual Vol. I TABLE 302-b Transmittal No. 2000-1 (10/1999) Form FDA 2905a (6/92) Chemical Recovery1,2 Eluant, C53 Notes4 Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19) diclobutrazol P 15 56-70% elution from Florisil only; 50% not tested; wide bore column recommended. dicloran C 15 dicofol, o,p'- C 15 dicofol, p,p'- C 15 May be variable. dieldrin C 15 difenoxuron NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. dimethachlor NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. dinobuton C (83%) 15 endosulfan I C 15 endosulfan II C 15 endosulfan sulfate C 15 Additional elution possible in 50% EE/PE. endrin C 15 esfenvalerate C 15 etaconazole S 15 30% elution from Florisil only; 50% not tested; wide bore column recommended. fenfuram P (45%) 15 Partial recovery also when Florisil eluted with MeCl2 eluant #3. fenpropathrin C 15 fenson C 15 fensulfothion sulfone NR 50 No elution from Florisil only in 50% EE/PE. fenvalerate C 15 flamprop-M-isopropyl NR 15 Complete elution from Florisil only in 50% EE/PE plus additional EE. flamprop-methyl NR 6-15-50 No elution from Florisil only in 6, 15, or 50% EE/PE; complete elution with EE. fluchloralin C 15 flucythrinate C 15+50 About 80% eluted in 15% EE/PE flusilazole S 15 35-44% elution from Florisil only; wide bore column recommended. fluvalinate C 15 FOE 5043 thioglycolate sulfoxide NR not eluted from Florisil folpet C 15+50 78% eluted in 15% EE/PE fonofos C 15
TABLE 302-b Pesticide Analytical Manual Vol I Table 302-b: Recovery Through 302 (E1-E3+ C5+DG1-DG19) Eluant. c53 fuberidazole NR No elution from Florisil only in 15%EE/PE heptachlor C Elution from Florisil not always complete heptachlor epoxide hexachlorobenzene Recovery test run with 6, 15, 50%EE/PE imazalil No elution from Florisil only in 15% EE/PE; 50% not tested. S(2497%)15 Complete elution from Florisil requires more olar eluants isocarbamid NR No elution from Florisil only in 15%EE/PE; 50% not tested lindane me methabenzthiazuron methoprotryne 5555b5 No elution from Florisil only in 15%EE/PE; 50% not tested. No elution from Florisil only in 15%EE/PE; 50% not tested. methyl 3, 5-dichlorobenzoate mirex myclobutanil No elution from Florisil only in 15%EE/PE 50% not tested nitrapyrin nitrothal-isopro CCcc nonachlor. trans- norflurazon Elution from Florisil with 50% EE/PE not ovex CCcPCc (79-96%)15 paclobutrazol 44-55% elution from Florisil only; 50% not tested; wide bore column recommended parathion parathion-methyl TABlE 302-b-44 Transmittal No. 2000-1 (1
TABLE 302-b Pesticide Analytical Manual Vol. I TABLE 302-b–4 Transmittal No. 2000-1 (10/1999) Form FDA 2905a (6/92) Chemical Recovery1,2 Eluant, C53 Notes4 Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19) fuberidazole NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. heptachlor C 15 Elution from Florisil not always complete. heptachlor epoxide C 15 hexachlorobenzene C 15 Recovery test run with 6,15,50% EE/PE, elution in 6%. hexaconazole NR 15 imazalil NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. iprodione S (24-97%) 15 Complete elution from Florisil requires more polar eluants. isocarbamid NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. lindane C 15 metalaxyl NR 15 methabenzthiazuron NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. methoprotryne NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. methyl 3,5-dichlorobenzoate C 15 mirex C 15 myclobutanil NR 15 No elution from Florisil only in 15% EE/PE; 50% not tested. nitrapyrin C 15 nitrofen C 15 nitrothal-isopropyl C 15 nonachlor, trans- C 15 norflurazon NR 15 Elution from Florisil with 50% EE/PE not tested. ovex C 15 oxadiazon C 15 oxythioquinox C (79-96%) 15 paclobutrazol P 15 44-55% elution from Florisil only; 50% not tested; wide bore column recommended. parathion C 15 parathion-methyl C 15