D Calcium imaging in ICC-MY from the guinea-pig antrum. A colocalization procedure was used to identify the rhod-2 signal from ACK2-Alexa 488 labelled ICC-MY Panel A shows a stack of 30 sequential optical sections made in the Z optical axis of the ACK2-Alexa 488 signal and the Rhod-2 signal panels B and C show each signal independently. Panel D shows the stack after the colocalization algorithm was used on each confocal slice, showing that most ICC-MY were well labelled with Rhod-2. It was evident from this experiment that some, but not all ICC-MY were well-labelled with rhod-2 see text for more details). The scale bar in panel d is 40 um and it applies to all images
Calcium imaging in ICC-MY from the guinea-pig antrum. A colocalization procedure was used to identify the Rhod-2 signal from ACK2-Alexa 488 labelled ICC-MY. Panel A shows a stack of 30 sequential optical sections made in the Z optical axis of the ACK2-Alexa 488 signal and the Rhod-2 signal; panels B and C show each signal independently. Panel D shows the stack after the colocalization algorithm was used on each confocal slice, showing that most ICC-MY were well labelled with Rhod-2. It was evident from this experiment that some, but not all, ICC-MY were well-labelled with Rhod-2 (see text for more details). The scale bar in panel D is 40 um and it applies to all images
B C 5mVL20sec Intracellularly recorded electrical activity from a guinea-pig antral ICC-MY identified with AcK2-Alexa 488 Panel A shows a network of ICc-MY labelled with AcK2-Alexa 488 visualized using fluorescence microscopy, and a single ICC MY impaled with a LY-filled microelectrode. Panel B shows changes in membrane potential recorded intracellularly from an ICC-MY. One slow wave. marked with the horizontal line in Panel B, is shown in Panel C at an expanded time scale. The scale bar is 15 um
Intracellularly recorded electrical activity from a guinea-pig antral ICC-MY identified with ACK2-Alexa 488. Panel A shows a network of ICC-MY labelled with ACK2-Alexa 488 visualized using fluorescence microscopy, and a single ICCMY impaled with a LY-filled microelectrode. Panel B shows changes in membrane potential recorded intracellularly from an ICC-MY. One slow wave, marked with the horizontal line in Panel B, is shown in Panel C at an expanded time scale. The scale bar is 15 um
B 165 160 150 145 140 135 130 50 t00 Time(sec Cyclic changes in intracellular calcium in ICC-MY in the murine jejunum Panel a shows a single confocal image with AcK2-Alexa 488 immunore activity (green) and Rhod-2 labelling(red) taken from a time series. ICC MY were distinctly labelled with Rhod-2 as shown in panel B. The average fluores cence intensity delineated by the white circled region was measured from images recorded every second shown in panel c
Cyclic changes in intracellular calcium in ICC-MY in the murine jejunum. Panel A shows a single confocal image with ACK2-Alexa 488 immunoreactivity (green) and Rhod-2 labelling (red) taken from a time series. ICCMY were distinctly labelled with Rhod-2 as shown in panel B. The average fluorescence intensity delineated by the white circled region was measured from images recorded every second, shown in panel C
Slow wave(basic electrical rhythm) ● Intensity:10~15mV ° Frequency:3~12cpm e lonic mechanism spontaneous rhythmic changes in Na*-K* pump activity
Slow wave (basic electrical rhythm) Intensity: 10~15 mV Frequency: 3~12 cpm Ionic mechanism spontaneous rhythmic changes in Na+ -K+ pump activity
Spikes 0 三三 若-10 三-20 Slow waves Depolarization 30 5-40 Stimulation by 50 1. Norepinephrine 60 Resting Stimulation b 2. Sympathetics E 1. Stretch 70 2. Acetylcholine 3. Parasympathetics Hyperolarization 061218243036424854 Seconds