分子生物学实验 PCR扩增目的基因
分子生物学实验 PCR扩增目的基因
实验目的 (1)PCR扩增目的基因红细胞生成因子nap基因 部分片段; (2)掌握PCR扩增原理 3)学习并熟悉PCR操作
实验目的 (1)PCR扩增目的基因红细胞生成因子nap基因 部分片段； (2)掌握PCR扩增原理； (3)学习并熟悉PCR操作
实验原理 (1)PCR反应原理 cells solate DNA DNA to be cloned sR合AB鼻合 CR AMPLIFICATION anomIc clones Figure 840 part 1 of 2. Molecular Biology of the cell, 4th Edition
实验原理 (1)PCR反应原理
+DNA polymerase HEAT TO DNA HYBRIDIZATION +dATP SEPARATE SYNTHESIS double-stranded OF PRIMERS +dGTP STRANDS FROM DNA +dCTP PRIMERS dTTP STEP 1 STEP 2 STEP 3 FIRST CYCLE Figure 8-39 part 1 of 3. Molecular Biology of the Cell, 4th Edition
(B) separate dNA strands and annea DNA separate dNA DNA primer synthesis strands and add prImer synthesis DNA oligonucleotide primers region of double-stranded chromosoma DNA to be amplified FIRST CYCLE SECOND CYCLE (producing two double-stranded (producing four double-stranded DNA molecules DNA molecules Figure 8-39 part 2 of 3. Molecular Biology of the Cell, 4th Edition