Transgenic Mice Famale Male Microinjection Pipette pronucleus pronucleus Holding DNA Solution Pipette Transgene Integration in the Genome Transgene Multiple copies integration at a random site in the genome Transfor t pseudopregnant female Q Transgenic mouse (transgene present in all nucleated cells)
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clone sheep Dol olly 体细胞提取核 重组细胞—回植— Dolly 卵细胞去核—细胞 Mammary Gland cells of 6 year old ewe Remove chromosomes Isolate and culture cells ↓ isolate ovulated oocytes Enucleated oocyte Culture in serum-depleted medium M Electric Cell growth Donor Go cells pulse 2 Inject into oocytes under zona pellucida ↓ Enucleated oocyte Transfer to foster owe
17 clone sheep Dolly 体细胞 提取 核 重组细胞 回植 Dolly 卵细胞 去核 细胞