An Introduction to Bioinformatics algorithms www.bioalgorithms.info Exhaustive Search and Branch-and-Bound Algorithms for Partial Digest Mapping
An Introduction to Bioinformatics Algorithms www.bioalgorithms.info Exhaustive Search and Branch-and-Bound Algorithms for Partial Digest Mapping
An Introduction to Bioinformatics algorithms www.bioalgorithms.info Molecular scissors EcoRI GHAATT C CTTAAIG Cleavage EcoRI Sticky ends 5 G AATT C 3 彐 CTTAA 5 Molecular Cell Biology edition
An Introduction to Bioinformatics Algorithms www.bioalgorithms.info Molecular Scissors Molecular Cell Biology, 4th edition
An Introduction to Bioinformatics algorithms www.bioalgorithms.info Recognition Sites of Restriction Enzymes Enzyme Source microorganism Recugnition Site Ends produces Baml Il Bacillus auryioligsefuciens -G-G-A-T-C-( Sticky (.(T-A(( 上cll Escherich coli GAATIC CTIAAG HindIll H若 aphis infima.e A- A-G-CI-I I-T-C-G.A-A- Kail (-(TA-(.C -C-C-A-T-C( Molecular Cell Biology edition
An Introduction to Bioinformatics Algorithms www.bioalgorithms.info Recognition Sites of Restriction Enzymes Molecular Cell Biology, 4th edition
An Introduction to Bioinformatics algorithms www.bioalgorithms.info Uses of Restriction Enzymes Recombinant DNA technology Cloning CDNA/genomic library construction DNA mapping
An Introduction to Bioinformatics Algorithms www.bioalgorithms.info Uses of Restriction Enzymes • Recombinant DNA technology • Cloning • cDNA/genomic library construction • DNA mapping
An Introduction to Bioinformatics algorithms www.bioalgorithms.info Restriction Maps A map showing positions of restriction sites in a DNA sequence Ec001092674 BstAP 179 If dNa sequence is Aatll 2617 Ndel 183 Sspl 2501 known then construction The 235 Pdml 2294 of restriction map is a BcgI 2215 MCS trivial exercise Scal 2177 In early days of molecular biology dNA sequences were often pUC18/19 Sapl 683 2686bp unknown Biologists had to solve Af/l, BspLU111 806 Gsul 1784 the problem of 1626 c011779 constructing restriction Ec0311766 Eam1105l1694 maps without knowing DNA sequences A Call 1217
An Introduction to Bioinformatics Algorithms www.bioalgorithms.info Restriction Maps • A map showing positions of restriction sites in a DNA sequence • If DNA sequence is known then construction of restriction map is a trivial exercise • In early days of molecular biology DNA sequences were often unknown • Biologists had to solve the problem of constructing restriction maps without knowing DNA sequences