Promoter -10sequence: TATAAT(Pribnow box): recognition site 35sequence: TTGACA is important in DNA unwind 35 16-18bp 10 +1 5′ MTTGACA TATAATMr Start site Promoters differ by up to 1000-fold in their efficiency of initiation of transcription
-10sequence:TATAAT(Pribnow box):recognition site -35sequence:TTGACA is important in DNA unwind 16-18bp 5-8bp Promoters differ by up to 1000-fold in their efficiency of initiation of transcription Promoter
Promoters and initiation C Exm宝主 mt主at主 L生重
Promoters and initiation
Transcription bubble Transcription bubble Nontemplate strand RNA polymerase Rewinding 5 Unwinding DNA 3′ 3 emplate strand RNA RNA-DNA Active site 5′ hybrid,8bp Direction of transcription (a)
Transcription bubble
Direction of transcription Negative supercoils A Positive RNA Direction of transcription (b)
Direction of transcription
Elongation strand o basebase .o base base H2 s
Elongation