C Enzymes C1 Introduction to enzymes C2 Thermodynamics热力学 C3 Enzyme kinetics动力学 C4 Enzyme inhibition C5 Requlation of enzyme activity
• C1 Introduction to enzymes • C2 Thermodynamics热力学 • C3 Enzyme kinetics动力学 • C4 Enzyme inhibition • C5 Regulation of enzyme activity C Enzymes
C1 Introduction to enzymes Enzyme as catalysts催化剂 Active site活性部位 Substrate specificity底物专一性 ° Enzyme classification分类 Enzyme assays酶的测定 Linked enzyme assays Coenzymes辅酶 and prosthetic groups辅基 Isoenzymes同工酶
• Enzyme as catalysts催化剂 • Active site 活性部位 • Substrate specificity底物专一性 • Enzyme classification分类 • Enzyme assays酶的测定 • Linked enzyme assays • Coenzymes辅酶 and prosthetic groups辅基 • Isoenzymes同工酶 C1 Introduction to enzymes
Enzymes as catalysts Enzymes are catalysts that change the rate of a reaction without being changed themselves Enzyme catalyzed reactions usually take place under relatively mild conditions(T, P,pH) Enzymes are highly specific and their activity can be requlated Virtually all enzymes are proteins, although some catalytically active RNAs have been identified
Enzymes as catalysts ➢ Enzymes are catalysts that change the rate of a reaction without being changed themselves ➢ Enzyme catalyzed reactions usually take place under relatively mild conditions(T,P,pH). ➢ Enzymes are highly specific and their activity can be regulated. ➢ Virtually all enzymes are proteins, although some catalytically active RNAs have been identified
Active site活性部位 Active site is the region that binds the substrate i and converts it into product 结合部位:负责与底物的结合,决定酶的专一性。 催化部位:负责催化底物键的断裂形成新键,决定酶的催 化能力。 Smal(1-2%)、 a three- dimensional entity实体 Cleft or crevice裂缝 on the surface of enzyme Bind by multiple weak forces Enzyme-substrate complex transition state complex过渡态复合物- product
Active site活性部位 • Active site is the region that binds the substrate 底物 and converts it into product。 • 结合部位：负责与底物的结合，决定酶的专一性。 • 催化部位：负责催化底物键的断裂形成新键，决定酶的催 化能力。 • Small（1-2%）、a three-dimensional entity实体 • Cleft or crevice裂缝on the surface of enzyme • Bind by multiple weak forces • Enzyme-substrate complex • transition state complex过渡态复合物--→product
Active site
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