Transcript Sense(+)strand=coding strand Antisense(-) strand=template strand 6)CGCTATAGCGTTT(8) DNA nontemplate( coding) strand
Sense(+) strand=coding strand Antisense(-) strand=template strand Transcript
RNA synthesis DNA 3′4 RNA RNA 5● 3 DNA promotor
5’ 3’ RNA 3’ 5’ DNA 3’ 5’ RNA 5’ 3’ DNA Promotor RNA synthesis
RNA polymerase p Subunit Core enzyme
RNA polymerase
RNA polymerase Subunit of rNa polymerase from Ecoli Subunit Gene Number Mass kd) Role 37 Binds regulatory sequences 1 151 Forms phosphodiester bonds 155 Binds DNA template d rpod 1 70 Recognizes promoter and nitrates synthesis
RNA polymerase
Promoter During initiation, RNA polymerases recognizes a specific site on the DNA, upstream from the gene that will be transcribed, called a promoter site 启动子是指RNA聚合酶识别、结合和开始转录的 段DNA序列
Promoter • During initiation,RNA polymerases recognizes a specific site on the DNA,upstream from the gene that will be transcribed,called a promoter site. • 启动子是指RNA 聚合酶识别、结合和开始转录的一 段DNA序列