Procaspase activation during apoptosis (1) procaspase activation active by cleavage caspase large NH2 NH2 subunit small subunit cleavage 111IN sites CLEAVAGE /1111 COOH COOH active inactive caspase prodomains procaspases 16 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
Procaspase activation during apoptosis (2) caspase cascade one molecule of active initiator caspase 11 /11N 11N /111 many molecules of executioner caspases cleavage of cleavage of cytosolic protein > nuclear lamin This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
The target proteins of caspase More than a dozen protein kinase,including FAK, PKC,and Raf1.FAK-disrupt cell adhesion for the apoptotic cell. >Lamins.Cleavage of lamins leads to the disassembly of the nuclear lamina and shrinkage of the nucleus. >Proteins required for cell structure.Such as IF, actin,and gelsolin.Cleavage and inactivation of these proteins lead to changes in cell shape. 18 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
4.2 Molecular pathways of apoptosis Two principle pathways: Extrinsic pathway:Fas Signaling Pathway -Intrinsic pathway:Mitochondrial pathway This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
The extrinsic pathway of apoptosis activated through Fas death receptors killer lymphocyte Fas ligand target cell Fas death receptor FADD 中 death domain adaptor DISC death effector activated protein domain caspase-8 or 10 death effector domain activation of procaspase-8 or 10 executioner caspases apoptotic ASSEMBLY ACTIVATION AND target cell OF DISC CLEAVAGE OF PROCASPASE-8,-10, OR BOTH 20 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information