安徽医科大学生命科学学院 School Of Life Sciences Aukui Wedieal Hniversity 细胞生物学实验之 微丝染色及形态观察 By生物学系-李超 2016-04 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[目的要求] 1.掌握微丝的染色方法。 2.了解光镜下微丝的基本形态结构。 3.了解考马斯亮蓝染料在细胞生物技术中的 应用。 2 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[实验原理] 1.细胞骨架 254m 25*m 25m INTERMEDIATE FILAMENTS MICROTUBULES ACTIN FILAMENTS 中间纤维 微管 微丝 3 鱼 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[实验原理] 2.微丝 肌动蛋白，actin 负端 G-actin 三维结构 细胞松弛素B CH2 H3Cx.. H H CH3 与0nm 正端 HN F-actin F-actin 电镜照片 分子模型 粉 4 座 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[实验原理] 3.染色 应力纤维 TritonX-100 考马斯亮蓝 处理 R250染色 ●溶解质膜和细胞内 ● 非特异性地将蛋白 许多蛋白质 染色 细胞骨架中蛋白质 ● 胞质背景着色弱 不被破坏 5 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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