Chapter 3. Techniques in Cell Biology Preparatory observe put forward theoretics Design control tests Refer to knowledge Collect data Explain results Devise conclusion
Chapter 3. Techniques in Cell Biology Preparatory observe put forward theoretics Design control tests Collect data Explain results Devise conclusion Refer to knowledge
从整个生命科学的发展趋势看细胞 生物学方法 分子水平 细胞水平 结构功能 细胞生命活动 分析 综合 功能基因组学研究是细胞生物学研究的 基础与归宿 (生命科学研究的核心问题)
从整个生命科学的发展趋势看细胞 生物学方法 • 分子水平 细胞水平 • 结构功能 细胞生命活动 • 分析 综合 • 功能基因组学研究是细胞生物学研究的 基础与归宿 （生命科学研究的核心问题）
细胞生物学研究方法 显微观篝 细胞培养 分子生物学技术 组纵学找术 胚胎学技术 生物化学找术 物理学技术
IThe light microscopy Ocular (eyepiece Remagnifies the image formed by the objectve lens Ocular lens Body tube Transmits the image from the ob- Line of vision jective lens to the ocular Path of light Objective lenses Primary Body tube lenses that magnify the Obiective Stage Holds the microscope slide in postion Condenser Focuses Condenser ragm Controis the amount entering the condenser Illuminator. Coarse focusing knob Luminator Light source source of Base allum nation Fine focusing knob (a)Principal parts and functions (b) The path of light(bottom to top) Figure A-6 The Compound Light Microscope. (a)A compound light microscope. (b)The path of light through the compound microscope
1.The Light Microscopy
A. Resolution and magnification animal Figure 3-1. Resolving power. Sizes 10 um of cells and their components drawn on a logarithmIc scale, indicating the range of objects that can be readily resolved by the naked eye and in the light and electron 100mm virus microscopes. The following units of length are commonly employed in globular microscopy: um(micrometer)=10 protein 6 mnm(nanometer)=10-9m A (Angstrom unit)=10-10 m molecule atom 0.1nr
Figure 3-1. Resolving power. Sizes of cells and their components drawn on a logarithmic scale, indicating the range of objects that can be readily resolved by the naked eye and in the light and electron microscopes. The following units of length are commonly employed in microscopy: µm (micrometer) = 10- 6 m nm (nanometer) = 10-9 m Å (Ångström unit) = 10-10 m