Chapter 7 Genetic Manipulation 第七章遗传操作 DNA manipulation DNA的遗传操作 o Basic techniques used to clone genes Construction and screening of genomic libraries The manipulation of cloned DNa sequences in vitro The molecular analysis of DNA Cell manipulation细胞的遗传操作 ◆ Human gene therapy ◆ Transgenic animals
Chapter 7 Genetic Manipulation 第七章 遗传操作 ◼ DNA manipulation DNA的遗传操作 ◆ Basic techniques used to clone genes ◆ Construction and screening of genomic libraries ◆ The manipulation of cloned DNA sequences in vitro ◆ The molecular analysis of DNA ◼ Cell manipulation 细胞的遗传操作 ◆ Human gene therapy ◆ Transgenic animals
DNA manipulation DNA的遗传操作 Recombinant DNA techniques 到的基的gn9o1n现 目的基因与载体结合成重组DIA 重组DNA分子引入受体细胞并表达 转化体细胞的扩增、鉴定与筛选
DNA manipulation DNA的遗传操作 Recombinant DNA techniques 目的基因(the gene of interest)的获取 目的基因与载体结合成重组DNA 重组DNA分子引入受体细胞并表达 转化体细胞的扩增、鉴定与筛选
Basic techniques used to clone genes The discovery of restriction endonucleases 限制性核酸内切酶 The production of recombinant DNA molecules in vitro Amplification of recombinant DNA molecules in cloning vectors
Basic techniques used to clone genes ✓ The discovery of restriction endonucleases 限制性核酸内切酶 ✓ The production of recombinant DNA molecules in vitro ✓ Amplification of recombinant DNA molecules in cloning vectors
Restriction endonucleases限制性核酸内切酶 限制与修饰作用 E coli K EOP=10-4 E OP=1 EOP=k 入K E coli B B 限制性核酸内切酶 restriction endonucleases
Restriction endonucleases 限制性核酸内切酶 ▪ 限制与修饰作用 ▪ 限制性核酸内切酶restriction endonucleases E.coli K E.coli B E.O.P = 1 E.O.P = 10-4 E.O.P = 1 λ .B λ .K
限制酶一能识别和切断外来的 DNA而使它失去复制能力 修饰酶一能使限制酶的识别序 列中的若干个碱基甲基化,限制酶 不能识别它
限制酶—能识别和切断外来的 DNA而使它失去复制能力 修饰酶—能使限制酶的识别序 列中的若干个碱基甲基化，限制酶 不能识别它