EcoRI cleavage sites DNA troT GAATTU 9AA丁了C DNA from Species I CTIAAG species 2 Digest with restrictio endonuclease EcoRI mT 3 ECoRI fragments CIAA TAA with complementary 53 5’ single-stranded ends Mix digested DNAS and incubate under annealing conditions. 5'f 3 Base-pairing between the complementary single-stranded ends of cleaved DNA molecules Treat annealed DNA fragments with DNA ligase DNA from iA DNA trom Species 1 3 Species 2 EcoRI cleavage site Recombinant DNA molecules
Recognition Sequences and Cleavage Sites of Representative Restriction Endonucleases NuImber of Recogmition Sequences per Recognition Sequence Chromosone o Enzie source an Cleavage Sites Plinge A sv40 Virus EcORI Escherichia coli AA TTO CTT AAG Hindi Herophilus influenzae GTPV PUACc CAPu PyTG HindIll Hemophilus influenzae AAG CTT TIC GAA p Hemophilus parainfluenzae GTT AAC CAA TTO Hpall Hemophilus parainfluenzae 750 GG CC