《遗传学》课程教学资源：（四）第三讲 分子细胞遗传学

分子细胞遗传学

分子细胞遗传学

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原位杂交( In situ hybridization) 1)同位素原位杂交( Isotopic in situ hybridization) 70年代 2)非同位素原位杂交( Non-isotopic in situ hybridization) 80年代中后期 (1)免疫酶联 (2)荧光原位杂交( Fluorescence in situ hybridization,FISH 六同位素原位杂交的不足之处: 1)不稳定; 2)低特异性; 3)高背景; 4)暴光时间长 5)结果统计学处理繁琐。 Lichter P. Cremert. Ward. c

原位杂交(In situ hybridization) 1）同位素原位杂交(Isotopic in situ hybridization) ——70年代 2）非同位素原位杂交(Non-isotopic in situ hybridization) ——80年代中后期 （1）免疫酶联 （2）荧光原位杂交（Fluorescence in situ hybridization, FISH) **同位素原位杂交的不足之处： 1）不稳定； 2）低特异性； 3）高背景； 4）暴光时间长； 5）结果统计学处理繁琐。 Lichter P. Cremer T. Ward D.C

FISH: Fluorescent In Situ Hybridization) FISH is the detection of highly specific dna probes which have been hybridized to either interphase or metaphase chromosomes using fluorescence microscopy. DNA for probe use is labeled with fluorescent(direct method)or non fluorescent molecules which are then detected by fluorescent antibodies (indirect method). The probes bind to a specific region or regions on the target chromosome. The chromosomes are then stained using a contrasting color, and the cells are viewed using a fluorescence microscope

FISH: (Fluorescent In Situ Hybridization). FISH is the detection of highly specific DNA probes which have been hybridized to either interphase or metaphase chromosomes using fluorescence microscopy. DNA for probe use is labeled with fluorescent (direct method) or non fluorescent molecules which are then detected by fluorescent antibodies (indirect method). The probes bind to a specific region or regions on the target chromosome. The chromosomes are then stained using a contrasting color, and the cells are viewed using a fluorescence microscope

Fluorescence In-situ Hybridization (FISH) chromosome on microscope slide denature A helix F probe labelled with TAGATCCT hybridize probe to: denatured denature hromosome TAGAT CCT TAG ATCCT 个TT个平个个个个 ATC TA G A