10. Sealable centrifuge buckets(safety cups)must be used for microorganisms in Risk Groups 3 and 4. I. When using angle-head centrifuge rotors, care must be taken to ensure that the tube is not overloaded as it might leak
10. Sealable centrifuge buckets (safety cups) must be used for microorganisms in Risk Groups 3 and 4. 11. When using angle-head centrifuge rotors, care must be taken to ensure that the tube is not overloaded as it might leak
12. The interior of the centrifuge bowl should be inspected daily for staining or soiling at the level of the rotor. If staining or soiling are evident then the centrifugation protocols should be re-evaluated. 3. Centrifuge rotors and buckets should be inspected daily for signs of corrosion and for hair-line cracks. 14. Buckets. rotors and centrifuge bowls should be decontaminated after each use 5. After use, buckets should be stored in an inverted position to drain the balancing fluid
12. The interior of the centrifuge bowl should be inspected daily for staining or soiling at the level of the rotor. If staining or soiling are evident then the centrifugation protocols should be re-evaluated. 13. Centrifuge rotors and buckets should be inspected daily for signs of corrosion and for hair-line cracks. 14. Buckets, rotors and centrifuge bowls should be decontaminated after each use. 15. After use, buckets should be stored in an inverted position to drain the balancing fluid
16. Infectious airborne particles may be ejected when centrifuges are used. These particles travel at speeds too high to be retained by the cabinet airflow if the centrifuge is placed in a traditional open-fronted Class I or Class ll biological safety cabinet. Enclosing centrifuges in Class ll safety cabinets prevents emitted aerosols from dispersing widely. However, good centrifuge technique and securely capped tubes offer adequate protection against infectious aerosols and dispersed particles
16. Infectious airborne particles may be ejected when centrifuges are used. These particles travel at speeds too high to be retained by the cabinet airflow if the centrifuge is placed in a traditional open-fronted Class I or Class II biological safety cabinet. Enclosing centrifuges in Class III safety cabinets prevents emitted aerosols from dispersing widely. However, good centrifuge technique and securely capped tubes offer adequate protection against infectious aerosols and dispersed particles
Use of homogenizers shakers, blenders and sonicator 1. Domestic (kitchen) homogenizers should not be used in laboratories as they may leak or release aerosols. Laboratory blenders and stomachers are safer 2. Caps and cups or bottles should be in good condition and free from flaws or distortion. Caps should be well fitting and gaskets should be in good condition
Use of homogenizers, shakers, blenders and sonicators 1. Domestic (kitchen) homogenizers should not be used in laboratories as they may leak or release aerosols. Laboratory blenders and stomachers are safer. 2. Caps and cups or bottles should be in good condition and free from flaws or distortion. Caps should be wellfitting and gaskets should be in good condition
3. Pressure builds up in the vessel during the operation of homogenizers, shakers and sonicator. Aerosols containing infectious materials may escape from between the cap and the vessel. Plastic, in particular, polytetrafluoroethylene (PTFE Vessels are recommended because glass may break, releasing infectious material and possibly wounding the operator
3. Pressure builds up in the vessel during the operation of homogenizers, shakers and sonicators. Aerosols containing infectious materials may escape from between the cap and the vessel. Plastic, in particular, polytetrafluoroethylene (PTFE) vessels are recommended because glass may break, releasing infectious material and possibly wounding the operator