Separation of serum 1. Only properly trained staff should be employed for this work. 2. Gloves and eye and mucous membrane protection should be worn 3. Splashes and aerosols can only be avoided or minimized by good laboratory technique. Blood and serum should be pipetted carefully, not poured Pipetting by mouth must be forbidden
Separation of serum 1. Only properly trained staff should be employed for this work. 2. Gloves and eye and mucous membrane protection should be worn. 3. Splashes and aerosols can only be avoided or minimized by good laboratory technique. Blood and serum should be pipetted carefully, not poured. Pipetting by mouth must be forbidden
4. After use, pipettes should be completely submerged in suitable disinfectant. They should remain in the disinfectant for the appropriate time before disposal or washing and sterilization for reuse. 5. Discarded specimen tubes containing blood clots, etc. (with caps replaced) should be placed in suitable leakproof containers for autoclaving and/or incineration 6. Suitable disinfectants should be available for clean-up of splashes and spillages
4. After use, pipettes should be completely submerged in suitable disinfectant. They should remain in the disinfectant for the appropriate time before disposal or washing and sterilization for reuse. 5. Discarded specimen tubes containing blood clots, etc. (with caps replaced) should be placed in suitable leakproof containers for autoclaving and/or incineration. 6. Suitable disinfectants should be available for clean-up of splashes and spillages
Use of centrifuges 1. Satisfactory mechanical performance is a prerequisite of microbiological safety in the use of laboratory centrifuges. 2. Centrifuges should be operated according to the manufacturer's instructions 3. Centrifuges should be placed at such a level that workers can see into the bowl to place trunnions and buckets correctly
Use of centrifuges 1. Satisfactory mechanical performance is a prerequisite of microbiological safety in the use of laboratory centrifuges. 2. Centrifuges should be operated according to the manufacturer’s instructions. 3. Centrifuges should be placed at such a level that workers can see into the bowl to place trunnions and buckets correctly
4. Centrifuge tubes and specimen containers for use in the centrifuge should be made of thick-walled glass or preferably of plastic and should be inspected for defects before use 5. Tubes and specimen containers should always be securely capped(screw-capped if possible) for centrifugation. 6. The buckets must be loaded, equilibrated, sealed and opened in a biological safety cabinet
4. Centrifuge tubes and specimen containers for use in the centrifuge should be made of thick-walled glass or preferably of plastic and should be inspected for defects before use. 5. Tubes and specimen containers should always be securely capped (screw-capped if possible) for centrifugation. 6. The buckets must be loaded, equilibrated, sealed and opened in a biological safety cabinet
7. Buckets and trunnions should be paired by weight and with tubes in place, correctly y balanced 8. The amount of space that should be left between the level of the fluid and the rim of the centrifuge tube should be given in manufacturers instructions. 9. Distilled water or alcohol (propanol, 70%) should be used for balancing empty buckets. Saline or hypochlorite solutions should not be used as they corrode metals
7. Buckets and trunnions should be paired by weight and, with tubes in place, correctly balanced. 8. The amount of space that should be left between the level of the fluid and the rim of the centrifuge tube should be given in manufacturer’s instructions. 9. Distilled water or alcohol (propanol, 70%) should be used for balancing empty buckets. Saline or hypochlorite solutions should not be used as they corrode metals