11. Safety equipment
11. Safety equipment
As aerosols are important sources of infection, care should be taken to reduce the extent of their formation and dispersion. Hazardous aerosols can be generated by many laboratory operations, e.g. blending, mixing, grinding, shaking, stirring, sonicating and centrifuging of infectious materials. Even when safe equipment is used, it is best to carry out these operations in an approved biological safety cabinet whenever possible
As aerosols are important sources of infection, care should be taken to reduce the extent of their formation and dispersion. Hazardous aerosols can be generated by many laboratory operations, e.g. blending, mixing, grinding, shaking, stirring, sonicating and centrifuging of infectious materials. Even when safe equipment is used, it is best to carry out these operations in an approved biological safety cabinet whenever possible
Biological safety cabinets and their use and testing are discussed in Chapter 10. The use of safety equipment is no assurance of protection unless the operator is trained and uses proper techniques. equipment should be tested regularly to ensure its continued safe performance
Biological safety cabinets and their use and testing are discussed in Chapter 10. The use of safety equipment is no assurance of protection unless the operator is trained and uses proper techniques. Equipment should be tested regularly to ensure its continued safe performance
Table 10 provides a checklist of safety equipment designed to eliminate or reduce certain hazards and briefly outlines the safety features. EQUIPMENT HAZARD CORRECTED SAFETY FEATURES Biological safety cabinet Class Aerosol and spatter Minimum inward airflow(face elocity) at work access opening Adequate filtration of exhaust air Does not provide product protection C|ass‖ Aerosol and spatter Minimum inward airflow(face velocity) at work access opening Adequate filtration of exhaust air Provides product protection Class Aerosol and spatter Maximum containment Provides product protection laminar flow air is included
Table 10 provides a checklist of safety equipment designed to eliminate or reduce certain hazards and briefly outlines the safety features
EQUIPMENT HAZARD CORRECTED SAFETY FEATURES Negative pressure Aerosol and spatter Maximum containment flexible-film isolator Spatter shield Spatter of chemicals Forms screen between operator and work Pipetting aids Hazards from pipetting by Ease of use mouth, e.g. ingestion of Controls contamination of suction pathogens, inhalation of end of pipette, protecting pipetting aerosols produced by mouth aid, user and vacuum line suction on pipette, blowing Can be sterilized out of liquid or dripping from Controls leakage from pipette tip pipette, contamination of suction end of pipette