Enlighten: found that a tri-nucleotide was usually enough like anmRNA to cause a specific aminoacyl-tRNAto bind to ribosomes氨基酰tRNUUU核糖体三核苷酸lArgUUUTalPheUUU
Enlighten：found that a tri-nucleotide was usually enough like an mRNA to cause a specific aminoacyl-tRNA to bind to ribosomes
Buta tri-nucleotide can not to causea synthesis of peptide!体外peptide合成系统方法进行改进利用不同polyNt指导peptide的合成(3个月内)Aug/1961-Nov/1961MarshallNirenberg两篇文章投稿<美国国家科学院院刊PNAS>遗传密码的破译找到了突破口
Aug/1961－Nov/1961 (3个月内) 两篇文章投稿 <美国国家科学院院刊 PNAS> 遗传密码的破译找到了突破口 Marshall Nirenberg 体外peptide合成系统方法进行改进 利用不同polyNt指导peptide的合成 But a tri-nucleotide can not to cause a synthesis of peptide !
Ser-c14, Leu, Lys, Arg,...UCUSer, Leu-Cl4, Lys, Arg....(trinucleotides)Ser, Leu, Lys-C14, Arg....tRNAaaRibosomeNitrocellulose filterM.Nirenberg &PLeder (1964.Science 145;1399)
M. Nirenberg & P. Leder (1964. Science 145;1399) tRNAaa Ribosome Nitrocellulose filter UCU (trinucleotides) SerC14 , Leu, Lys, Arg,. Ser, Leu-C14, Lys, Arg,. Ser, Leu, Lys-C14 , Arg,.
Ser-C14Lys-C14Leu-C14Gly-C141?C14C14UCUSer!
Lys-C14. Leu-C14 . Ser-C14 . Gly-C14 . C14 C14 UCU Ser !
20(saunua)pungoAAA15AAG10AGA5GAA51015O20Trinucleotide added (nmol)Binding of c14lys-tRNA to ribosomesAAA, AAG coding Lys
Binding of C14lys-tRNA to ribosomes AAA，AAG coding Lys