23s rRNA--- 6 domains有的与对抗生素的抗性有关--- 2660±Nt region α - I loop(alpha Sarcin)binding with complex of aa-tRNAaa~(EF)-Tu~GTP（引起核糖体变构！！）G2661 → C, aa-tRNAaa into A site go downG2253双突变为C,对转肽酶的活性产生抑制--J22529
● 23s rRNA - 6 domains - 有的与对抗生素的抗性有关 - 2660±Nt region α - I loop(alpha Sarcin) binding with complex of aa-tRNAaa~(EF)-Tu~GTP (引起核糖体变构！！) G2661 C , aa-tRNAaa into A site go down - G2252, G2253双突变为C, 对转肽酶的活性产生抑制
In Euk.与原核生物高度相似3'-end of 18s rRNA但无与S.D.seq.互补的保守序列mRNA的AUG上游存在ccACC核糖体scanningseq成为核糖体识别第一个AUG的信号BacterialAMEAMECCUGCGGUUGGAUGACCUCCUU16SMammalian18SAMEAMECCUGCGGAAGGAUGAUUA高度相似
In Euk. 3’-end of 18s rRNA 与原核生物高度相似 但无与 S.D.seq.互补的保守序列 mRNA的AUG上游存在CCACC核糖体scanning seq 成为核糖体识别第一个 AUG 的信号 AMEAMECCUGCGGUUGGAUGACCUCCUU AMEAMECCUGCGGAAGGAUGAUUA 16S Bacterial 18S Mammalian 高度相似
Biad ribosomatomtiaticnsitecnmRNA5.2.3mRNAAUGIn ProkaryoteAad nuclease pdigest allniotectedmRNA+++++poly-cistron5'-end; 300 ±Nt leading se. (A/GAUG)Shine-Dalgarno seq. (S.D seg) GGAGGAUGS.D seq7-9Nt betterG mut.translation go downrich A,U
5.2.3 mRNA ● In Prokaryote 5’-end; 300±Nt leading seq. (A/G-AUG) S.D seq-AUG poly-cistron 7－9Nt better rich A,U, Shine-Dalgarno seq. (S.D seq) GGAGG G mut. translation go down
In Eukaryotemono-cistronleading seq.CCACC--5'm7Gppp---A.3---A,U,G3G4核糖体小亚基扫描AUG至关准确翻译的信号序列But: polycistron in Euk. of yeasthavebeenidentified!
● In Eukaryote mono-cistron 5’ m7Gppp- -CCACC-A-3 -A1U2G3G4— leading seq. 核糖体小亚基扫描AUG 的信号序列 至关准确翻译 But : polycistron in Euk. of yeast have been identified !
5.3Genetic Code
5.3 Genetic Code