、G⑧》一e 9 Ring opening hk 0 +H=-caH=aH-G= NH 5-0, DNA fragmnen国 0P=0 -O4 DNA fragment
●TOA+G we can weaken the glycosidic bonds to both adenine and guanine with acid;then piperidine will cause depurination and strand breakage after both A's and G's
⚫TO A+G ⚫ we can weaken the glycosidic bonds to both adenine and guanine with acid; then piperidine will cause depurination and strand breakage after both A's and G's
●TOC+T ●Similarly,hydrazine(肼)opens both thymine and cytosine rings,and piperidine can then remove these bases and break the DNA strand at the resulting apyrlmidinic sites. ●TOC In the presence of 1 M NaC1,hydrazine is specific for cytosine only,so we can run this reaction next to the C+T reaction and obtain the T's by comparison
⚫TO C+T ⚫ Similarly, hydrazine(肼）opens both thymine and cytosine rings, and piperidine can then remove these bases and break the DNA strand at the resulting apyrlmidinic sites. ⚫TO C ⚫ In the presence of 1 M NaC1, hydrazine is specific for cytosine only, so we can run this reaction next to the C + T reaction and obtain the T's by comparison
Thymine O CH, CH NHg-一N再 Hydrxrine OH H oIr 0 OH HO 5-P04 DNA fragment 0 家-P04 DNA fragment
Maxam-Gilbert sequencing is used only in special cases these days,but the methods of modifying and then breaking DNA used by Maxam a and Gilbert are still useful for other purposes
Maxam-Gilbert sequencing is used only in special cases these days, but the methods of modifying and then breaking DNA used by Maxam and Gilbert are still useful for other purposes