Standalone and APl blast Download BLAST Use BLAST API Use BLAST in the cloud Get blast databases and executables Call BLAST from your application Specialized searches SmartBLAST Primer-BLAST Global Align CD-search Find proteins highly similar Design primers specific to Compare two sequences Find conserved domains in your PCR template across their your sequence (Needleman-Wunsch) GEO IgBLAST Vecscreen CDART Find matches to gene Search immunoglobulins Find sequences with similar expression profile contamination conserved domain sequences architecture Targeted Loci Multiple alignment BioAssay MOLE-BLAST Search markers for Align sequences using Establish taxonomy for phylogenetic analysis domain and protein nucleotide targets in uncultured or enviromental
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Blast种类 blastp protein protein Use blastp to compare a protein query to a database of proteins DNA DNA blastn Use blastn to compare both strands of a DNa query against a DNA database. DNA blastx protein Blastx translates a DNA sequence into six protein sequences using all six possible reading frames, and then compares each of these proteins to a protein database protein tblastn Tblastn is used to translate every DNa sequence in a database into six potential proteins and then to compare your protein query against each of those translated proteins DNA DNA tblastx Tblastx is the most computational intensive BLAST algorithm. It translates DNA from both a query and a database into six potential proteins, then performs 36 protein-protein database searches
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> Blast种类选择 Basic Local Alignment Search Tool NBLAST+2.4.released BLAST finds regions of similarity between biological sequences. The program E Anew version (2.4.0) f the BLAST executable is now available compares nucleotide or protein sequences to sequence databases and Thu,02Jun201614:00:00EST A More blast news alculates the statistical significan Learn more Web blast blastx translated nucleotide b protein ● Nucleotide BLAST tblastn Protein BLAST nucleotide b nucleotid protein b translated nucleotide protein ) protein BLAST Genomes Enter organism common name, scientific name, or tax id Search Human M Microbes
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U.S. National Library of Medicine NCBI National Center for Biotechnology Information Sign in to NCBI BLAST > blastn suite Home Recent Results Saved Strategies Help Standard Nucleotide BLAST m)Bst种类 Reset page Bookmark Enter Query Sequence BLASTN programs search nucleotide databases using a nucleotide query. more Enter accession number(s), gi(s), or FASTA sequence(s)e Query subrange >lcllM251131 cds AAA3596611 [protein=sickle beta-hemoglobin] Iprotein id=AAA359661] [location=1.444] UTGGIGCACCTGACTCCIGIGGAGAAGICYGCNGITACTGCNYINTGGGGCAAGGGAACGTGGATGA To TTGGTGGTGAGGCCCGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTccTT Or, upload file 输入序列 lob titl IM25113 1 cds AAA359661 1 (protein=sickle Enter a descriptive title for your BLAST search e L Align two or more sequences o Choose Search Set Database OHuman genomic +transcript MOuse genomic +transcript O Others (nr etc) Nucleotide collection(nrint) √9 Organism 口 Exclude Enter organism common name, binomial, or tax id Only 20 top taxa will be shown e Exclude D Models(XMXP) Uncultured/environmental sample sequences Optional Limit L Sequences from type material Entrez Query You ube Create custom database Enter an Entrez query to limit search g Program Selection e Highly similar sequences(megablast) O More dissimilar sequences(discontiguous megablast) O Somewhat similar sequences(blastn) Choose a BLAST algorithm e BLAST)Search database Nucleotide collection (nr/nt) using Megablast(Optimize for highly similar sequences) L Show results in a new window
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U5 National Ubrary of Medicine NCBI National Center for Biotechnology informaton sEn an to NCB BLAST Home Recent Results Saved Strategies Help Format Request Job Title: IclM25113,1 cds AAA359661 1 Dproteinssicke Request D PD6SC1N7015 Time since submission 000000 Ths page will be automatically updated in 1 seconds untl search is done BLAST m a reeled trodmmak od the Natiral Leran tif Mtdrne NCBI National Center for Blotechnology infornation sigint NCEl BLAST blastn suite M RID-PDBD5S9UOIR Home Recent Results Saved Strategies Help BLAST Results Edit and Resubmit Save Search Strategies bEormaning options >Download You- How to read this page Biast report descnption IclM25113 1 cds AAA35966, 1 1 [proteinsickle RID PDBD559 01B(Expires on 0620 22: 35 pm) Database Name nr escription ld M251131_cds_AAA35966, 1-1 Iprotein=sickie beta.-hernoglobinI Description Nudeotide collection(nt) [protein d AAA35966 1][location-1. 444) ASTN 2.4.0+ potation Molecule type nucleic acid Query Length 444 mary TAxonomy reports [Distance tree of results + Graphic Summary +Descriptions +)Alignments
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