高分子传感材料一六 Fluorescent DNA-based enzyme sensors Molecular Engineering of DNA: Molecular Beacons Biological Applications of Mole cular Beacons
高分子传感材料—六 Fluorescent DNA-based enzyme sensors Molecular Engineering of DNA: Molecular Beacons Biological Applications of Molecular Beacons
2 Molecular Engineering of DNA: Molecular Beacons 分子信标 Molecular Beacons的基本构成是怎样的? 2分子信标的工作原理是什么? 31分子信标设计的基本原则有哪些? Complementary Target
1 分子信标的工作原理是什么？ Molecular Engineering of DNA: Molecular Beacons 分子信标 (Molecular Beacons) 的基本构成是怎样的？ 2 3 分子信标设计的基本原则有哪些？
3 Biological Applications of Mole cular Beacons 实时检测PcR过程 Real-time PCR assays 2探测三股DNA( Detection of triplex DNA 31单核酸多态性与基因筛选( SNP and Genetic Screening 4监测蛋白质 Monitoring proteins) 5生物传感器与生物芯片( Biosensors and biochips)
Biological Applications of Molecular Beacons 1 单核酸多态性与基因筛选 (SNP and Genetic Screening) 2 探测三股DNA(Detection of triplex DNA) 实时检测PCR过程 (Real-time PCR assays) 3 4 监测蛋白质(Monitoring proteins) 5 生物传感器与生物芯片 (Biosensors and biochips)
34监测蛋白质 Monitoring proteins MB SSB Application of MBs in enzymatic Binding、Q 号埠 studies a) Real-time monitoring of SSB-DNA binding: The MB binds to the ssb protein Cleavage whereby its structure is MB Enzyme disrupted and its fluorescence is Cutting restored b)detection of the enzymatic o digestion of DNA: The enzyme cleaves the mb and destroys the hairpin structure to restore MB LDH fluorescence Binding c)Detection of LDH-DNA x interactions: LDH binds the MB oo and disturbs its structure whereby fluorescence is enhanced
3.4 监测蛋白质(Monitoring proteins) Application of MBs in enzymatic studies. a) Real-time monitoring of SSB–DNA binding: The MB binds to the SSB protein whereby its structure is disrupted and its fluorescence is restored. b) Detection of the enzymatic digestion of DNA: The enzyme cleaves the MB and destroys the hairpin structure to restore fluorescence. c) Detection of LDH–DNA interactions: LDH binds the MB and disturbs its structure, whereby fluorescence is enhanced
34监测蛋白质 Monitoring proteins Detect Protein-DNA Interactions:o Molecular Beacons: A Novel approac Jianwei Jeffery Li, Xiaohong Fang, Sheldon M. schuster, and Weihong Tan s Prof, W. Tan, Dr JJ. Li, Dr. X. Fang, Prof S.M. Schuster TGC Department of Chemistry, Biochemistry and Molecular Biology and uf Brain Institute University of Florida 0=P-O Angew. Chem. Int. Ed. 2000, 39, No. 6 Q b) Q Q TAMRA DABCYI MB
3.4 监测蛋白质(Monitoring proteins)