安徽医科大学生命科学学院 School Of Life Sciences Aukui Wedieal Hniversity 细胞生物学实验之 细胞器的分级分离 By生物学系-李超 2016-04 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[目的要求] 1.初步掌握细胞器的分离原理及方法。 2.学习并掌握高速离心机和匀浆器的使 用方法。 3.掌握差速离心法和密度梯度离心法的 原理。 2 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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Cell Membrane Centrosome 细 Lysosome】 别 Cyteplasm [实验原理] Nucleus Rough ER Smeoth ER Nuclear 1.细胞器的分离 Membrane 制备细 分级 组分分 胞匀浆 分离 析鉴定 ● 机械法 ● 差速离心法 ●形态鉴定 ●物理法 ● 密度梯度离心法 ●功能鉴定 ● 化学法 3 鱼 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[实验原理] 2.分级分离方法 差速离心法(Differential centrifugation) 速度区带离心法 (Rate-zone 心方 密度梯度离心法 centrifugation) (Density gradient centrifugation) 等密度区带离心法 法 (Isopycnic density gradie nt zonal centrifugation) 超速离心法(Jltracentrifugation) 静 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[实验原理] 2.分级分离方法 (1)差速离心法(Differential centrifugation) SUPERNATANT 1 SUPERNATANT 2 SUPERNATANT 3 LOW-SPEED MEDIUM-SPEED HIGH-SPEED VERY HIGH-SPEED CENTRIFUGATION CENTRIFUGATION CENTRIFUGATION CENTRIFUGATION 的 cell homogenate PELLET 1 PELLET 2 PELLET 3 PELLET 4 whole cells mitochondria microsomes ribosomes nuclei lysosomes other small vesicies viruses cytoskeletons peroxisomes large macromolecules 完整细胞、细胞核、 线粒体、溶酶 微体、囊泡等 核糖体、病毒等 细胞骨架 体、过氧化物 酶体 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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