安徽医科大学生命科学学院 Sckool Aike Scicnees Anbui medial Uaiwersity 细胞生物学实验之 碱性蛋白与酸性蛋白 的显示 By生物学系-李超 2016-04 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[目的要求] 1.学会制作血涂片。 2.了解碱性蛋白与酸性蛋白的显示方法的 原理及操作步骤。 3.了解碱性蛋白与酸性蛋白的分布。 2 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
This document is produced by trial version of Print2Flash. Visit www.print2flash.com for more information
[实验原理] 以酸性氨基酸成分为 主的蛋白质为酸性蛋白；以 碱性氨基酸成分为主的蛋白 质为碱性蛋白。细胞核内有 组蛋白（碱性蛋白）及少量 酸性蛋白，细胞质中主要有 酸性蛋白，标本经三氯醋酸 处理抽提掉核酸后，用不同 pH值的快绿染液分别染色 使细胞内的酸性蛋白和碱性 蛋白质显示出来。 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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本期实验内容 蟾蜍心包血细胞中碱性蛋白的显示 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
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[实验用品] 材料：蟾蜍(or牛蛙)血涂片 器具：普通光学显微镜、恒温水浴箱、载玻片、染色 缸等。 试剂：70%乙醇溶液，5%三氯醋酸溶液，0.2%快绿染 液，0.005%Na2CO3溶液，碱性蛋白染色液（pH8.0): 0.2%快绿和等量0.005%Na2C03溶液混合而成。 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more information
This document is produced by trial version of Print2Flash. Visit www.print2flash.com for more information