(: Capping protein ce=cr Capping protoin C 2 GG-actin concentration >L 8一
ADPATP actin with actin with bound ADP bound ATP
●o Whole cilium 8 ontains axonemes with Pellet Supernal (contains axoneme (contains outer with missing outer Incubate with EDTA 8 supernatant Pellet (contains outer fibers) in presence of Me' 8 m activity is present oluble fraction Figure 9.38 Steps in the chemical dissection of cilia
tubulin molecule C with bound GTP GTP tubulin molecules a protofilaments containing GDP add to end of microtubule tubulin are unstable and peel away from the microtubule wall addition proceeds faster GDP tubulin is released than GTP hydrolysis to the cytosol 翻鞲、` tubulin molecule with bound GDP GTP cap GROWING MICROTUBULE SHRINKING MICROTUBULE
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