Agarose gel electrophoresis Agarose: a polysaccharide derived from seaweed which forms a solid gel when dissolved in aqueous solution(0.5%-2% Negatively charged DNA Power supply (up to 150 V DC) DNA sample Migration of DNA Agarose gel Conducting buffer solution ve electrode ve electrode Fig. 3. Agarose gel apparatus
Agarose: a polysaccharide derived from seaweed, which forms a solid gel when dissolved in aqueous solution (0.5%-2%) - ve electrode + ve electrode Negatively charged DNA Agarose gel electrophoresis
Agarose gel electrophoresis MU B E M (b) 140 120 Marker E100 E supercoiled c 66 Migration of DNA g 芝60 10 10 DNA length(bp) 056
supercoiled nicked Agarose gel electrophoresis
DNA ligation Covalently join the DNA molecules with the base-pairing cohesive ends, or blunt ends. if the 5 -ends lave phosphate groups
Covalently join the DNA molecules with the base-pairing cohesive ends, or blunt ends, if the 5’-ends have phosphate groups. DNA ligation