◇ The quaternary structure consists of six subunits of a protein 16
16 The quaternary structure consists of six subunits of a protein
Techniques used to produce biotechnologic products ◇·1. Recombinant dna 2. Monoclonalantibodies 3. Polymerase chain reaction 4. Gene therapy ◇ 5. Nucleotide blockade/antisense 6. Peptide technology 17
17 Techniques used to produce biotechnologic products • 1. Recombinant DNA • 2. Monoclonal Antibodies • 3. Polymerase Chain Reaction • 4. Gene therapy • 5. Nucleotide Blockade/ Antisense • 6. Peptide technology
1. Recombinant dna Recombinant dna is a form of dna that s does not exist naturally. which is created combining dna sequences that would not normally occur together Joining two different dna molecules together to get Recombinant DNA (rDNA) 18
18 1.Recombinant DNA • Recombinant DNA is a form of DNA that does not exist naturally, which is created by combining DNA sequences that would not normally occur together . • Joining two different DNA molecules together to get Recombinant DNA (rDNA)
o. The ablility to join two different pieces of dna at specific sites within the molecules is achieved with ◇ two enzymes, a restriction endonuclease(内切酶) and a dNa ligase(连接酶) With rDNA technology, scientists can use nonhuman cells to manufacture proteins identical to ◇ those produced in human cells
19 • The ablility to join two different pieces of DNA at specific sites within the molecules is achieved with two enzymes, a restriction endonuclease(内切酶) and a DNA ligase(连接酶). • With rDNA technology, scientists can use nonhuman cells to manufacture proteins identical to those produced in human cells
重组DNA技术 ·重组DNA技术( recombinant dna technique)又称遗传工程,在体外重新 组合脱氧核糖核酸(DNA)分子,并使 ◆它们在适当的细胞中增殖的遗传操作。 这种操作可把特定的基因组合到载体上, 并使之在受体细胞中增殖和表达。 20
20 重组DNA技术 • 重组DNA技术（recombinant DNA technique）又称遗传工程，在体外重新 组合脱氧核糖核酸（DNA）分子，并使 它们在适当的细胞中增殖的遗传操作。 • 这种操作可把特定的基因组合到载体上， 并使之在受体细胞中增殖和表达