Kinetics: similar to MichaeliS-Menten equation omn×7k k2 k3 T out S:. T S+T k-2 D-Glucose Vmax「 Sout Kt+ [ sin KtKm for affinity of transporter for substrate ④ Inside Outside
Kinetics: similar to Michaelis-Menten equation Sout + T Sout . T Sin . T Sin + T V0 = Kt~Km for affinity of transporter for substrate k1 k-1 k2 k-2 k3 k-3 Vmax [S]out Kt + [S]in
max Kinetics Initial velocity plot s 2 max Kt Extracellular glucose concentration, [Slout(mM) (a)
Kinetics: Initial velocity plot
Double-reciprocal plot max 1 SI out mM K
Double-reciprocal plot
D-Glucose Model of glucose Transport into erythrocytes by glut1 Step① 1) Binding to TI site 2)Conformational change from TI to t2 3)Released 4)Return of conformation to T Kt for GluT1=1. 5mM for D-glucose R Step@ 20-30 mM for D-mannose and d Outside galactose, >3000mM for L-glucose (fast, saturable and specific)
Model of Glucose Transport into erythrocytes by GluT1 1) Binding to T1 site 2) Conformational change from T1 to T2 3) Released 4) Return of conformation to T1 Kt for GluT1=1.5mM for D-glucose, 20-30 mM for D-mannose and Dgalactose, >3000mM for L-glucose (fast, saturable and specific)
GluT2: transport glucose out of hepatocytes to blood, Kt=66mM(5mM of blood glucose eve GluT4: for muscle, adipocytes, can be stimulated by insulin
GluT2: transport glucose out of hepatocytes to blood, Kt=66mM (>5mM of blood glucose level) GluT4: for muscle, adipocytes, can be stimulated by insulin