1、 Properties of HBV a member of the hepadnavirus group Circular partially double-stranded DNA Viruses Replication involves a reverse transcriptase endemic in the human population and hyperendemic in many parts of the world a number of variants It has not yet been possible to propogate the virus in cell culture
1、Properties of HBV • a member of the hepadnavirus group • Circular partially double-stranded DNA viruses • Replication involves a reverse transcriptase. • endemic in the human population and hyperendemic in many parts of the world. • a number of variants • It has not yet been possible to propogate the virus in cell culture
HBV. Structure Copynght e The McGraw-Hill Companies, Inc. Permission required for reproduction or display Genomic dNA (double-stranded with partial single strand Hepatitis Envelope B surface antigen (HBsAg) Nucleocapsid (viral capsid) Spherical Envelope lipid (from host cell) Hepatitis B e antigen Hepatitis B core antigen Elongated (a)Complete infectious virion (b)Viral envelope particles containing HBSAg
HBV : Structure
HBV. Structure Virion also referred to as Dane particle(ds-tranded DNA 42nm enveloped virus Core antigens located in the center(nucleocapsid Core antigen(HBCAg) e antigen(HBeAg)-an indicator of transmissibility (minor component of the core-antigenically distinct from HBCAg) 22nm spheres and filaments other forms-no dNA in these forms so they are not infectious(composed of surface antigen)-these forms outnumber the actual vIrions
HBV : Structure • Virion also referred to as Dane particle (ds-tranded DNA) • 42nm enveloped virus • Core antigens located in the center (nucleocapsid) * Core antigen (HBcAg) * e antigen (HBeAg)- an indicator of transmissibility (minor component of the core- antigenically distinct from HBcAg) • 22nm spheres and filaments other forms- no DNA in these forms so they are not infectious (composed of surface antigen)- these forms outnumber the actual virions
HBV Structure Antigens Dane particle Hepatitis B surface antigen M DNA polymerase DNA (mostly double stranded), 3200 bp A Core antigen Protein kinase 42 nm ZAll/I/MMW HBsAg= surface(coat protein(4 phenotypes: adw, adr, ayw and ayr) HBCAg inner core protein(a single serotype HBeAg=secreted protein; function unknown
HBV Structure & Antigens Dane particle HBsAg = surface (coat) protein ( 4 phenotypes : adw, adr, ayw and ayr) HBcAg = inner core protein (a single serotype) HBeAg = secreted protein; function unknown
decoy particles HBSAg- containing particles are released into the serum of infected 22 nm people and outnumber the actual virions Spherical or filamentous They are immunogenic and were processed into the first commercial vaccine against HBV. 100-700m
decoy particles • HBsAg-containing particles are released into the serum of infected people and outnumber the actual virions. • Spherical or filamentous • They are immunogenic and were processed into the first commercial vaccine against HBV