《培养基及其成分146种》资料讲义

month of bottle. Park the bottle with paper. Autoclave at 20 lbs for lhr.(将10x2x1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水, 装瓶不宜过紧,用纸包好,20磅灭菌1小时。) 2. Pine sawdust medium Pine sawdust(松木屑)75% Rice bran(米糠)23-24% Sucrose(蔗糖)1-2% Water( appropriate)水(适量 Autoclave at20 lbs for 1 hr.(20磅灭菌1小时) 23. Rice straw. Dung Medium(稻草、马粪培养基 Dried horse dung( or cow dung)(干马粪或牛粪)65% Died straw(干稻草)35%Urea(尿素)0.5% Calcium superhosphate(过磷酸钙)0.7% Gypsum powder(石膏粉)1% Ammonium sulphate(硫酸铵)0.3% [Note]: Add appropriate water, pile them up and cause them fermentation (水适量进行堆积发酵) 24. Straw rice bran medium(稻草、米糠培养基) Dried straw(干稻草)75-80% Rice bran(米糠)20-25% [Note]: Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drol of water appeared between fingers).([注]:选无霉稻草,切成长约3厘米 的小段,用清水浸泡12小时,使充分吸水,然后倾去水,加米糠和水,水量以 用手捏时指间有水珠为宜。) 25. Rice medium(米饭培养基) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml wate Autoclave at 121C for 30 minutes (or fill 2g of rice into a 15 X 150mm tube).Add6 ml of water. Autoclave at12l℃for30 minutes.(取大米 10克,入50毫升椎形瓶中,水20-30毫升于15磅灭菌30分钟即成,或取大米 2克,装入15ⅹ150毫米试管中,加水6毫升,于15磅灭菌30分钟。) 26. Filter Paper Medium(滤纸条培养基) (NH4)2S04 1g KH2P04 1g MgS04.7H200.5 g Yeast Extract(酵母膏)0.1g Distilled Water(蒸馏水)1000ml Strip of filter paper1x7cm(滤纸条1x7厘米) [Note]: Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains Iml of the broth). Autoclave at 121C for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically.(将上述溶液分配成溶液后分装入试管,每管1毫升,15磅灭菌 30分钟。再将灭菌的滤纸条(1条或2条)投入试管中即好使用。) 27. Yeast Extract- Medium Extract Agar I(酵母膏、麦芽膏琼脂)

month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将 10ⅹ2ⅹ1 厘米的松木条浸泡在 1-2%的蔗糖水中。使木条吸足糖水， 装瓶不宜过紧，用纸包好，20 磅灭菌 1 小时。) 2. Pine Sawdust Medium Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24% Socrose (蔗糖) 1-2% Water (appropriate)水 （适量） Autoclave at 20 lbs for 1 hr.(20 磅灭菌 1 小时） 23. Rice Straw. Dung Medium (稻草、马粪培养基) Dried horse dung (or cow dung) （干马粪或牛粪） 65% Died straw （干稻草） 35% Urea （尿素） 0.5% Calcium superhosphate （过磷酸钙）0.7% Gypsum powder（石膏粉） 1% Ammonium sulphate （硫酸铵） 0.3% [Note]: Add appropriate water, pile them up and cause them fermentation. （水适量进行堆积发酵） 24. Straw Rice Bran Medium （稻草、米糠培养基） Dried straw （干稻草） 75-80% Rice bran （米糠） 20-25% [Note]：Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]：选无霉稻草，切成长约 3 厘米 的小段，用清水浸泡 12 小时，使充分吸水，然后倾去水，加米糠和水，水量以 用手捏时指间有水珠为宜。) 25. Rice Medium (米饭培养基) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米 10 克，入 50 毫升椎形瓶中，水 20-30 毫升于 15 磅灭菌 30 分钟即成，或取大米 2 克，装入 15ⅹ150 毫米试管中，加水 6 毫升，于 15 磅灭菌 30 分钟。) 26. Filter Paper Medium (滤纸条培养基) (NH4)2SO4 1g KH2PO4 1g MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g Distilled Water (蒸馏水) 1000ml Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米) [Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (将上述溶液分配成溶液后分装入试管，每管 1 毫升，15 磅灭菌 30 分钟。再将灭菌的滤纸条（1 条或 2 条）投入试管中即好使用。) 27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)

Yeast extract(酵母膏)10 g Mall extract(麦芽膏)10g Glucose(葡萄糖)4 g Distilled water(蒸馏水)1000m1 Agar(琼脂)20gpH7.0 适用范围:红霉素链霉菌 28. Yeast Extract Peptone(酵母膏、蛋白胨琼脂) Yeast extract(酵母膏)1 g Multi- peptone(多蛋白胨)2g Beef extract(牛肉膏) Ig glucose(葡萄糖)10g Agar(琼脂)20 g Distilled water(蒸馏水)1L pH7.0 29. Glucose Yeast Extract Starch Agar(葡萄糖、酵母、淀粉琼脂) Glucose(葡萄糖)10 g Yeast extract(酵母膏)10g Starch(淀粉)10 g NaCl5g CaC033 g Agar(琼脂) Distilled water(蒸馏水)1LpH7.0 30. Wheat bran medium(麸皮培养基) Wheat bran(麸皮)36g(NH)2HPO410g K2HP04 0 2g MgS04. 7H20 0. lg Agar(琼脂)20 g Distilled water(蒸馏水)1L 31. Yeast Sporulation Medium(酵母菌产生子囊孢子培养基) (1)Kleyn Medi KH2PO40.012%K2HPO40.02% Sodium acetate(醋酸钠)0.5% Glucose(葡萄糖)0.062% NaC10.062% Peptone(蛋白胨)0.25% Biotin(生物素)2ug%Agar(琼脂)2% Trace element solution(混合盐类)1ml/100m1 [Note]: Trace element solution(混合盐类溶液):MgS04.7H200.4%,NaC 0.4%,CuS04.5H200.002%,MnS04.4H200.2%,FeS04.4H200.2% (2)Gorodkowa Medium Glucose(葡萄糖)0.1% Peptone(蛋白胨)1% NaC10.5%Agar(琼脂)2% (3)McClary Medium Glucose(葡萄糖)0.1%KC10.18% Yeast juice(酵母汁)0.25% Sodium actate(醋酸钠)0.82% Agar(琼脂)1.5% 32. BPY Medium(BPY培养基) Beef extract(牛肉膏)5 g Peptone(蛋白胨)10g NaCl5 g Yeast extract(酵母膏)5g Glucose(葡萄糖)5 g Agar(琼脂)1-2%

Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml Agar (琼脂) 20g pH 7.0 适用范围：红霉素链霉菌 28. Yeast Extract Peptone （酵母膏、蛋白胨琼脂） Yeast extract （酵母膏） 1g Multi-peptone（多蛋白胨） 2g Beef extract （牛肉膏） 1g Glucose （葡萄糖） 10g Agar （琼脂） 20g Distilled water （蒸馏水） 1L pH 7.0 29. Glucose Yeast Extract Starch Agar （葡萄糖、酵母、淀粉琼脂） Glucose （葡萄糖） 10g Yeast extract （酵母膏） 10g Starch （淀粉） 10g NaCl 5g CaCO3 3g Agar （琼脂） 20g Distilled water （蒸馏水） 1L pH 7.0 30. Wheat Bran Medium （麸皮培养基） Wheat bran （麸皮） 36g （NH4）2HPO4 10g K2HPO4 0.2g MgSO4。7H2O 0.1g Agar （琼脂） 20g Distilled water （蒸馏水） 1L pH 7.0 31. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基) (1) Kleyn Medium KH2PO4 0.012% K2HPO4 0.02% Sodium acetate (醋酸钠) 0.5% Glucose (葡萄糖) 0.062% NaCl 0.062% Peptone (蛋白胨) 0.25% Biotin (生物素) 2μg% Agar (琼脂) 2% Trace element solution (混合盐类) 1ml/100ml [Note]: Trace element solution （混合盐类溶液）： MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2% (2) Gorodkowa Medium Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1% NaCl 0.5% Agar (琼脂) 2% (3) McClary Medium Glucose (葡萄糖) 0.1% KCl 0.18% Yeast juice (酵母汁) 0.25% Sodium actate (醋酸钠) 0.82% Agar (琼脂) 1.5% 32. BPY Medium (BPY 培养基) Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g NaCl 5g Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g Agar (琼脂) 1-2%

Distilled(蒸馏水)1000mlpH7.0 适用范围:大肠埃希氏菌(大肠杆菌)、恶臭假单胞菌 33. LB Medium(LB培养基) Yeast extact(酵母膏)5 g Peptone(蛋白胨)10g NaCl10 g Agar(琼脂)1-2% Distilled water(蒸馏水)1000mlpH7.0 适用范围:大肠埃希氏菌(大肠杆菌) 34. Penssay Broth( Penssay肉汤) Beef extract(牛肉膏)1.5 g Yeast extract(酵母膏)1.5g Tryptone(胰蛋白胨)5gNaC13.5g Glucose(葡萄糖)1gK2HPO4.3H204.8g Distilled water(蒸馏水)1000m1pH7.2 35. Blond Agar(血琼脂培养基) Peptone(蛋白胨)l0 g Glucose(葡萄糖)2g Nacl 5g NaH2P04 2 5g Sodium citrate(柠檬酸钠)5 g Brain infusion(脑浸汁)800r Pig heart infusion(猪心浸)200 mI Sleep blood(羊血)10% pH7.2 [Note]: Preparation of pig brain and pig heart infusion: (1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml )Cut 200g fresh pig brain(without blood vessel). Add distilled water o1000ml; (3) Keep the two infusions at 50C for 1 hr, then boil for 15 minutes (4) Cool and filtrate through gauze.[注]猪心和猪脑浸汁法:(1)新 鲜猪心除去心头和脂肪,搅碎100克加蒸馏水至1000毫升;(2)新鲜猪脑除去 血管,取200克加蒸馏水至1000毫升;(3)50℃保温1小时,然后煮开15分 钟;(4)冷却后用纱布过滤备用。 36. Cellulose- decomposing Bacteria Synthetic Medium(纤维细菌合成培养 基) Nacl 6g MgS04. 7H20 0. lg KH2P040.5gCaC120.1 K2HPO42.0 g Yeast extract(酵母膏)1.0g (NH4)2S042.0 g Distilled water(蒸馏水)1000ml Filter paper strip: one strip(滤纸条:一片)pH7.0-7.4 Note]: So; id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose2%Agar%.(斜面培养基去滤纸条用2%琼脂及2%羧甲基纤维素钠 CMCNa

Distilled (蒸馏水) 1000ml pH 7.0 适用范围：大肠埃希氏菌（大肠杆菌）、恶臭假单胞菌 33. LB Medium (LB 培养基) Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g NaCl 10g Agar (琼脂) 1-2% Distilled water (蒸馏水) 1000ml pH 7.0 适用范围：大肠埃希氏菌（大肠杆菌） 34. Penssay Broth (Penssay 肉汤) Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g Tryptone (胰蛋白胨) 5g NaCl 3.5g Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g Distilled water (蒸馏水) 1000ml pH 7.2 35. Blond Agar (血琼脂培养基) Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g NaCl 5g NaH2PO4 2.5g Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10% pH 7.2 [Note]：Preparation of pig brain and pig heart infusion： （1） Smash up 100g fresh pig heart （without fat and blood vessel）.Add distilled water up to 1000ml; （2） Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml; （3） Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes; （4） Cool and filtrate through gauze . [注]猪心和猪脑浸汁法：（1）新 鲜猪心除去心头和脂肪，搅碎 100 克加蒸馏水至 1000 毫升；（2）新鲜猪脑除去 血管，取 200 克加蒸馏水至 1000 毫升；（3）50℃保温 1 小时，然后煮开 15 分 钟；（4）冷却后用纱布过滤备用。 36. Cellulose﹣decomposing Bacteria Synthetic Medium (纤维细菌合成培养 基) NaCl 6g MgSO4.7H2O 0.1g KH2PO4 0.5g CaCl2 0.1g K2HPO4 2.0g Yeast extract (酵母膏) 1.0g (NH4)2SO4 2.0g Distilled water (蒸馏水) 1000ml Filter paper strip: one strip (滤纸条：一片) pH 7.0-7.4 [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. （斜面培养基去滤纸条用 2%琼脂及 2%羧甲基纤维素钠 CMCNa ）

37. Clostridium Pasreurianum Synthetic Medium(巴氏梭状芽孢杆菌合成培 养基) Glucose(葡萄糖)1%MnS040.001% KH2P040.05%FeSO40.001% K2HPO40.05% Yeast extract(酵母膏)0.1% MgS04.7H200.02% Peptone(蛋白胨)0.01% NaC10.001%CaC030.5% Distille water(蒸馏水)100%pH7.0 38. Yeast Extract- Medium Extract AgarⅡl(酵母膏、麦芽膏琼脂Ⅱ) Yeast extract(酵母膏)4 g Mall extract(麦芽膏)10g Glucose(葡萄糖)4 g Agar(琼脂)17g Distilled water(蒸馏)1000m1pH7.3 39.0 atemeal Agar(燕麦粉琼脂) Oatmeal( infusion)[燕麦粉浸液]20 g Agar(琼脂)18% Trace element solution(迹量盐溶液)1ml Distilled water up to(用蒸馏水补充至1000毫升)1000mlpH7.2 INote]: Trace element solut 迹量盐溶液) FeS04 7H20 0. lg MnC12. 4H20 0. lg ZnS04.7H200. l g distilled water(蒸馏水)100m1 40. Calcium Malate Agar(苹果酸钙琼脂) Calium malate(苹果酸钙)10gK2HPO40.5g NH4C10.5 g Glycerol(甘油)10 Agar(琼脂)15 g Distilled water(蒸馏水)1000ml 41. Thiobacillus ferrooxidans medium(氧化亚铁硫杆菌培养基) (NH4)2S040.15gKH2PO40.05g KC10.05gMgS04.7H200.5g Ca(NO3)2.4H200.01gFeS04.TH2050g Distilled water(蒸馏水)1000mlpH2.0 42. Thiobacillus Thiooxidans medium(氧化硫硫杆菌培养基) (NH4)2S040.3gKH2PO43-4g CaC12( anhydrous)[无水]0.25gMgS04.7H200.5g FeS04.7H200.00 Ig Distilled water(蒸馏水)1000m1 pH3.5-4 43. Plasma Substitute medium(代血浆培养基) Sucrose(蔗糖)12.5-15% Peptone(蛋白胨)0.5% KH2PO40.03%Na2HP04.12H200.14% pH7.0-7.2

37. Clostridium Pasreurianum Synthetic Medium （巴氏梭状芽孢杆菌合成培 养基） Glucose （葡萄糖） 1% MnSO4 0.001% KH2PO4 0.05% FeSO4 0.001% K2HPO4 0.05% Yeast extract（酵母膏） 0.1% MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01% NaCl 0.001% CaCO3 0.5% Distille water (蒸馏水) 100% pH 7.0 38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麦芽膏琼脂 Ⅱ) Yeast extract （酵母膏） 4g Mall extract （麦芽膏） 10g Glucose （葡萄糖） 4g Agar （琼脂） 17g Distilled water （蒸馏） 1000ml pH 7.3 39. Oatemeal Agar （燕麦粉琼脂） Oatemeal（infusion）[燕麦粉浸液] 20g Agar （琼脂） 18% Trace element solution （迹量盐溶液）1ml Distilled water up to （用蒸馏水补充至 1000 毫升） 1000ml pH 7.2 [Note]：Trace element solution (迹量盐溶液)： FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g ZnSO4.7H2O 0.1g Distilled water (蒸馏水) 100ml 40. Calcium Malate Agar (苹果酸钙琼脂) Calium malate (苹果酸钙) 10g K2HPO4 0.5g NH4Cl 0.5g Glycerol (甘油) 10g Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml 41. Thiobacillus Ferrooxidans Medium (氧化亚铁硫杆菌培养基) (NH4)2SO4 0.15g KH2PO4 0.05g KCl 0.05g MgSO4.7H2O 0.5g Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g Distilled water (蒸馏水) 1000ml pH 2.0 42. Thiobacillus Thiooxidans Medium (氧化硫硫杆菌培养基) (NH4)2SO4 0.3g KH2PO4 3-4g CaCl2 (anhydrous) [无水] 0.25g MgSO4.7H2O 0.5g FeSO4.7H2O 0.001g Distilled water (蒸馏水) 1000ml pH 3.5-4 43. Plasma Substitute Medium (代血浆培养基) Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5% KH2PO4 0.03% Na2HPO4.12H2O 0.14% pH 7.0-7.2