a 1-2 Site-specific recombinases cleave and a rejoin DNA using a covalent protein-DNA 后 intermediate Serine Recombinases : Tyrosine Recombinases cleaved dNA end 5-070 ase cleavage H Rec. Ser-OH 0 ase Rec.Ser-0-P-o base er-OH Fiqure 11-5 protein-DNA covalent intermediate
◼ Serine Recombinases ◼ Tyrosine Recombinases Conservative Site -Specific Recombination 1-2 Site-specific recombinases cleave and rejoin DNA using a covalent protein-DNA intermediate Figure 11-5
1-3 Serine recombinases introduce a double-stranded breaks in DNA and then swap strands to promote recombination
Conservative Site -Specific Recombination 1-3 Serine recombinases introduce double-stranded breaks in DNA and then swap strands to promote recombination
crossover re recombinase recognition sites ecombinase RI R2 H R-3 R4 swap DNA partners 多 OH 3 R1 R4 H3 Fiqure 11-6 combinate
Conservative Site-Specific Recombination Figure 11-6
1-4 Tyrosine recombinases break and rejoin one pair of dNa strands at a time
Conservative Site -Specific Recombination 1-4 Tyrosine recombinases break and rejoin one pair of DNA strands at a time
SOH R R3 SOH R4 op strand R1 多 Holliday junction R2 P R4 cleavage of DoRTon strands RT 多 R s。 RA Figure 11-7 bottom strand ecchange to fina recombinaton
Figure 11-7