a 1-1 Site-specific recombination occurs at specific DNA sequences in the target DNA CSSR(conserved site-specific recombination) is responsible o for many reactions in which a 8 defined segment of DNa is rearranged
◼ CSSR (conserved site-specific recombination) is responsible for many reactions in which a defined segment of DNA is rearranged. Conservative Site -Specific Recombination 1-1 Site-specific recombination occurs at specific DNA sequences in the target DNA
SSR can generate three different types of DNA rearrangements insertion deletion inversion Fiqure 11-3
CSSR can generate three different types of DNA rearrangements Figure 11-3
If the two sites at which recombination will take place are oriented oppositely to one another in the same DNA molecule then the site-specific reacom bination results in inversion of the segment of dna between the two recombination sites X A recombination at inverted repeats causes ah version
If the two sites at which recombination will take place are oriented oppositely to one another in the same DNA molecule then the site-specific reacombination results in inversion of the segment of DNA between the two recombination sites recombination at inverted repeats causes an inversion
If the two sites at which recombination will take place are oriented in the same direction in the same DNA molecule, then the segment of DNA between the two recombinogenic sites is deleted from the rest of the DNA molecule and appears as a circular molecule. Insertion is the reverse reaction of the deletion recombination at direct repeats causes a deletion
If the two sites at which recombination will take place are oriented in the same direction in the same DNA molecule, then the segment of DNA between the two recombinogenic sites is deleted from the rest of the DNA molecule and appears as a circular molecule. Insertion is the reverse reaction of the deletion recombination at direct repeats causes a deletion
recombination sites recombinase recognition sequences Fiqure 11-4 crossover region Structures involved in recombinase binds CSSR recognition sequences recombination c G
Figure 11-4 Structures involved in CSSR