The re are two ty pes of me thy lase Three types of DNA/AvAN GC Methylation enzymes De novo methylase De novo dna CG methyltransferases GC Perpetuation methylase Perpetuation DNA VVAVA人 GC methyltransferases M Demethylase DNA demethylase VAVAVA AVAVAN Ge wArtuglkex w. roto. com 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 26 Three types of DNA Methylation enzymes De novo DNA methyltransferases Perpetuation DNA methyltransferases DNA demethylase
Three types of reaction could TExt w.e n demethylate DNA Removal of Removal of Removal of methyl group 5-Me-C base nucleotide 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 27 Three types of reaction could demethylate DNA
Embryo Methyl groups NT公地 halLe Methylation is Reset each ACTIVE Patemal Allele generation Gametes of next generation Females give eggs Males give sperm Me 八八八 M avirtualtextwwcrgito.com Ity
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 28 Methylation is Reset each generation
DNA甲基化转移酶 ■DNA甲基化转移酶 类甲基化转移酶Dnmt1,Dmmt2,Dmmt3a/3b Dnmt1在DNA复制时维持DNA甲基化。 ● Dnmt2可能与DNA上特异位点结合,但具体作 用还不是很清楚; ●Dnmt3的功能可能主要是对着丝粒卫星DNA的 重复序列起甲基化作用,但 Dnmt3a,Dnmt3b 作用并不重叠。 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 29 DNA甲基化转移酶 DNA甲基化转移酶 三类甲基化转移酶 三类甲基化转移酶 Dnmt1, Dnmt2,Dnmt3a/3b Dnmt1, Dnmt2,Dnmt3a/3b ● Dnmt1在DNA复制时维持DNA甲基化。 ● Dnmt2可能与DNA上特异位点结合，但具体作 上特异位点结合，但具体作 用还不是很清楚 用还不是很清楚; ● Dnmt3的功能可能主要是对着丝粒卫星 的功能可能主要是对着丝粒卫星DNA的 重复序列起甲基化作用，但 重复序列起甲基化作用，但Dnmt3a, Dnmt3b Dnmt3a, Dnmt3b 作用并不重叠
Mammalian methyltransferases DNMT1-maintenance DNA methyltransferase methylates hemi-methylated dNA providing methylation pattern to the newly replicated daugther strand, based on parent strand represses transcription in complex with histone deacetylases 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 30 Mammalian methyltransferases: DNMT1 - maintenance DNA methyltransferase — methylates hemi-methylated DNA providing methylation pattern to the newly replicated daugther strand, based on parent strand — represses transcription in complex with histone deacetylases