廳遗 Epigenetics Chromatin based gene control Chaoqun Wu School of Life Sciences, Fudan University 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 1 Epigenetics — Chromatin based gene control Chaoqun Wu School of Life Sciences, Fudan University
Part‖l Chromatin has important role in gene regulation 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 2 Part II. Chromatin has important role in gene regulation
Multiple levels of gene regulation inactive mRNA NUCLEUS CYTOSOL mRNA degradation 5 contro RNA DNA transcript mRNA mRNA transcriptional RNA RNA control processing transport translation protein control and control activity local ization control contro protein In nactive protein 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 3 Multiple levels of gene regulation
RNA polymerase transcribes genes How is this process regulated? How is specificity achieved? Key players transcriptional activator proteins bind to specific sites on dNA and turn on the expression of nearby genes Two adhesive surfaces one binds dna. the other interacts with and recruits RNa polymerase 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 4 RNA polymerase transcribes genes - How is this process regulated? How is specificity achieved? Key players – transcriptional activator proteins -bind to specific sites on DNA and turn on the expression of nearby genes. Two adhesive surfaces – one binds DNA, the other interacts with, and recruits RNA polymerase
Enzyme specificity Many enzymes are capable of acting on a common motif found in many different proteins-EXample -kinases General solution recruitment through specific protein protein interactions Regulated localization -locator proteins 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 5 Enzyme specificity – Many enzymes are capable of acting on a common motif found in many different proteins - Example - kinases General solution – recruitment through specific proteinprotein interactions. Regulated localization - locator proteins