Negative control, dimer binding to OR1 inhibits RNAp binding to right Pr promoter Negative control, dimer binding to OR1 inhibits RNAp binding to left PRM promoter(too distant Image removed due to copyright considerations See Ptashne, Mark. A genetic switch: phage lambda 3rd ed. Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press, 2004
II Negative control, dimer binding to OR1 inhibits RNAp binding to right PR promoter. Negative control, dimer binding to OR1 inhibits RNAp binding to left PRM promoter (too distant). Image removed due to copyright considerations. See Ptashne, Mark. A genetic switch: phage lambda. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004
Negative control, dimer binding to OR3 inhibits RNAp binding to left PRM promoter Positive control, dimer binding to OR3 allows RNAp binding to right PR promoter Image removed due to copyright considerations See Ptashne, Mark. A genetic switch: phage lambda 3rd ed. Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press, 2004
III Negative control, dimer binding to OR3 inhibits RNAp binding to left PRM promoter. Positive control, dimer binding to OR3 allows RNAp binding to right PR promoter. Image removed due to copyright considerations. See Ptashne, Mark. A genetic switch: phage lambda. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004
Repressor-DNa binding is highly cooperative trinsic association constants 10K 10 However KoR2 > KoR2(positive cooperativity) Image removed due to copyright considerations See Ptashne, Mark. A genetic switch: phage lambda 3rd ed. Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press, 2004
Repressor-DNA binding is highly cooperative intrinsic association constants: KOR1 ~ 10 KOR2 ~ 10 KOR3 However KOR2* >> KOR2 (positive cooperativity) Image removed due to copyright considerations. See Ptashne, Mark. A genetic switch: phage lambda. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004