The three major identified nucleosome remodeling atPases Swi2/Snf2 ISwI Mi-2 ATPase ATPase ATPase III■工口 Br SANT-domain Chromodomains homologues/ isoforms homologue/ Isoforms homologues Sth1 (in RSC complex) yeast iswi and lsw2 in yeast CHD1-4, Hs D3 and 4 are BRG1 and hBRM in human hSNF2H, hSNF2L In human synonyms for N2aandβ Brahma in Drosophila IswI In Drosophila found in found in found in SWUSNF complex NURF CHRAC, ACF NURD related complexes RSC (Sth1) (Drosophila In frog and human and complexes In Drosophila ISw1 and 2 complex in yeast and human RSF, hACF, WCRF, HUCHRAC In human ISwI complexes A-D in frog 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 26 The three major identified nucleosome remodeling ATPases
Mammalian ATPase-dependent chromatin-remodeling complexes Mammalian ATPa8- dependent chromatin-Memodel“的cmpx SNF2-ike subunit Complex F SNF2- e subfamily BRG1, BRM, SMARO31 BRG1, BRM, bBAF, hRSC/PBAF, WNAC Transcription, cedl-cyde progresson, and DNA replicafon KSW↓k鲁 subfamily hswi(n2hhsnta) ACF/BAZ1A, CHRAC, ASF, NoPC, WICH Transarlptian, DNA rapicafon CHD subfamily CHDS. CHD4 NURO DNArepair and methMladonnduoed gene slencng and heterochromatin o山 r SNF2 related ATRX ATRX ERCC8 CSB DNA repair and transcription SMARCAL1 Unknown Unknown (Current Opinion in Genetics Development 2004, 14: 308-315 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 27 Mammalian ATPase-dependent chromatin-remodeling complexes (Current Opinion in Genetics & Development 2004, 14:308–315)
Representative members of the SWl-sNF ISWI and mi-2/CHd subclasses of chromatin remodeling enzymes that are found in human cells hSWI/SNF h CHRAC hMi-2/NURD MBD3 BAF hAcK Mi-2 BAF250 Brgl MTA、CHD34 BAF155 BAF17O hSNF2H RbA MTAI hISuI BAFA RbA BAF HDAC2 46 50 Subclass: SWI/SNF Mi-2/CIID Role Transcription activation Chromatin Assembly Transcription repression Transcription repression? Operation: Nucleosome mobilization Nucleosome mobilization Nucleosome mobilization Nucleosome disruption Histone deacetylation Delivery: Gene-specific activators Replication factors? cific repressors A Braking Linker histone Linker histone Linker histone PRCI complex 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 28 Representative members of the SWI–SNF, ISWI and Mi-2/CHD subclasses of chromatin remodeling enzymes that are found in human cells
The three major identified nucleosome remodeling aTPases, their domains homologues, and complexes in which they are found Each atPase core domain has seven, highly conserved subdomains. This ATPase core-domains are braced by domains that differ considerably between the three types of nucleosome remodeling ATPases 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 29 The three major identified nucleosome remodeling ATPases, their domains, homologues, and complexes in which they are found. Each ATPase core domain has seven, highly conserved subdomains. This ATPase core-domains are braced by domains that differ considerably between the three types of nucleosome remodeling ATPases
H4 tall Mi-2 Histone cores ISWI DNA and histone tails SWI/SNF DNA Interactions of nucleosome and some proteins in nucleosome-remodeling complexes 2005-10-29 Chaoqun Wu, Fudan University
2005-10-29 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 30 Interactions of nucleosome and some proteins in nucleosome-remodeling complexes