光学成像原理 Objective Lens” Focal length “Object Plane” “Image Plane” -u Magnified Light Object is placed image win 1 and 2fof Source the objective lens 1st image 1 1 + and M=3 f 11
光学成像原理 11
Magnification in Compound Microscope (transmission illumination) Final image projected Condenser Objective onto a aperture aperture screen or Object Projector film (specimen) lens Objective lens First image Final image B ￠ Light Source Condenser lens vl v2 M=M,M,=出-)-) f Total mag.is product of mags.for each lens. 4 12
12
日m b' 人眼所看到 Eye 的像 Eyepiece 目镜 b 九元汽气5气三艺三三 Fo Objective 物镜 ob Specimen 日◆
人眼所看到 的像 13 25 cm 物镜 目镜
放大倍率与分辨率 ·放大倍率 一人眼从显微镜目镜中所见的被观察物尺寸与原 物尺寸的比值。 一人眼明视距离除以物镜焦距。 分辨率 一区分两个相邻点的能力 ·放大倍率上限？ 14
放大倍率与分辨率 • 放大倍率 – 人眼从显微镜目镜中所见的被观察物尺寸与原 物尺寸的比值。 – 人眼明视距离除以物镜焦距。 • 分辨率 – 区分两个相邻点的能力 • 放大倍率上限？ 14
人眼的分辨率 μ=133 Object μ1 Image 4=1.38 Iris is a variable μ=1.38-1.41 aperture that adjusts intensity of Optic nerve detects signal light allowed in. projected onto retina by curved lens. ~0.2 mm 25 cm distance 15
人眼的分辨率 15 ~0.2 mm @ 25 cm distance