The covalent protein-DNA intermediate conserves the energy of the cleaved phosphodiester bond within the protein-DNA linkage, which allows the cleaved dna strands to be rejoined/resealed by reversal of the the cleavage process This mechanistic feature contributes the conservative to the CSSR name, because every dNa bond that is broken during the reaction is resealed by the recombinase without consuming any external energy
The covalent protein-DNA intermediate conserves the energy of the cleaved phosphodiester bond within the protein-DNA linkage, which allows the cleaved DNA strands to be rejoined/resealed by reversal of the the cleavage process This mechanistic feature contributes the “conservative” to the CSSR name, because every DNA bond that is broken during the reaction is resealed by the recombinase without consuming any external energy
1-3 Serine recombinases introduce 2 double-stranded breaks in dna and then swap strands to promote recombination 03595
Conservative Site -Specific Recombination 1-3 Serine recombinases introduce double-stranded breaks in DNA and then swap strands to promote recombination
crossover region recombinase recognition sites 零 serIne rec。 kinase cleavage R1 R之 OH 3 3 Hc R3 R4 OH 3 swap DNA partners R3 零 R2 2 OH 3 R4 当 B·H R1 OH 3. Fic gure 11-6 rejoining serine recombinase
Conservative Site-Specific Recombination Figure 11-6
1-4 Tyrosine recombinases break and a rejoin one pair of DNA strands at a time 03595
Conservative Site -Specific Recombination 1-4 Tyrosine recombinases break and rejoin one pair of DNA strands at a time
3P-tyrosine R19 5。H 尺3 S. OH R4 strand hange RT Holiday junction R3 c-O R4 cleavage of bottom strands RT 多 R之 H SOH R4 Figure 11-7 bottom strand exchange to finish recombination 3
Figure 11-7