CSSR can generate three different types of DNA rearrangements insertion dell inversion X A Figure 11-3
CSSR can generate three different types of DNA rearrangements Figure 11-3
If the two sites at which recombination will take place are oriented oppositely to one another in the same DNA molecule then the site-specific recombination results in inversion of the segment of dna between the two recombination sites A A recombination at inverted repeats causes an inversion
If the two sites at which recombination will take place are oriented oppositely to one another in the same DNA molecule then the site-specific reacombination results in inversion of the segment of DNA between the two recombination sites recombination at inverted repeats causes an inversion
If the two sites at which recombination wil take place are oriented in the same direction in the same DNA molecule, then the segment of dNA between the two recombinogenic sites is deleted from the rest of the dNA molecule and appears as a circular molecule, insertion is the reverse reaction of the deletion recombination at direct repeats causes a deletion
If the two sites at which recombination will take place are oriented in the same direction in the same DNA molecule, then the segment of DNA between the two recombinogenic sites is deleted from the rest of the DNA molecule and appears as a circular molecule. Insertion is the reverse reaction of the deletion recombination at direct repeats causes a deletion
recombination sites recombinase recognition sequences Figure 11-4 crossover region Structures 535 involved in ICG recombinase binds CssR recognition sequences 5353 ATCG c recombination IAGG=
Figure 11-4 Structures involved in CSSR
1-2 Site-specific recombinases cleave and 3 rejoin ( ioin) DNa using a covalent protein S DNA intermediate Serine Recombinases Q. Tyrosine Recombinases cleaved DNA end 835 5-0 base 5-0 5-0 cleavage HQ 0 Red Ser-oH 0 Ser 0 0=P-0 Ser-OH O 0 Figure 11-5 protein-DNA covalent intermediate
◼ Serine Recombinases ◼ Tyrosine Recombinases Conservative Site -Specific Recombination 1-2 Site-specific recombinases cleave and rejoin (join) DNA using a covalent proteinDNA intermediate Figure 11-5