RNA干扰技术的应用马欣荣
RNA干扰技术的应用 马欣荣
RNAiVirus inhibition and gene silencingCo-suppression and transposon silencingPosttranscriptiongenesilencingTranscription genesilencingTransgenic gene silencingmRNAcleavageDNA methylationsiRNAsmRNAtranslation(?)Polycombsuppression(?)
Transgenic gene silencing
高通量的研究基因功能基因敲除geneknockout,knockdown基因治疗gene therapeutics基因表达调控
➢ 高通量的研究基因功能 ➢ 基因敲除 gene knock out, knock down ➢ 基因治疗 gene therapeutics ➢ 基因表达调控
一、基因功能研究1. RNAihas be a powerful tool with which todissect gene function in plant, C.elegansDrosophila,and other lower eukaryoteorganismsSpecificand heritablegeneticinterferencebydouble-stranded RNA inArabidopsisthaliana,Chiou-Fen Chuang and Elliot M.Meyerowitz,PNAS,April25,2000,vol.97no9,4985-4990
一、基因功能研究 1. RNAi has be a powerful tool with which to dissect gene function in plant, C.elegans , Drosophila, and other lower eukaryote organisms Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana，Chiou-Fen Chuang and Elliot M. Meyerowitz， PNAS, April 25, 2000, vol.97, no 9, 4985-4990
RNAi技术研究了拟南芥的AGAMOUS（AG),CLAVATA3 (CLV3),APETALA1 (AP1)PERIANTHIA（PAN）四个开花相关基因
RNAi 技术研究了拟南芥的AGAMOUS (AG) , CLAVATA3 (CLV3), APETALA1 (AP1), PERIANTHIA (PAN) 四个开花相关基因