Mesosome invagination of the plasma membrane in the shape of vesicles, tubules, or lamellae Functions 1. cell wall formation during division 2. play a role in chromosome replicaton and distribution to daughter cells
Mesosome: invaginationa of the plasma membrane in the shape of vesicles, tubules, or lamellae. Functions: 1.cell wall formation during division. 2.play a role in chromosome replicaton and distribution to daughter cells
Making Wall-less forms Result from action of -enzymes lytic for cell wall -antibiotics inhibiting peptidoglycan biosynthesis Wall-less bacteria that dont replicate -spheroplasts (with outer membrane)? protoplasts(no outer membrane).? Wall-less bacteria that replicate -L forms Naturally Wall-less Genus Mycoplasma
Making Wall-less forms • Result from action of: –enzymes lytic for cell wall –antibiotics inhibiting peptidoglycan biosynthesis • Wall-less bacteria that don’t replicate: –spheroplasts (with outer membrane)? –protoplasts (no outer membrane). ? • Wall-less bacteria that replicate –L forms Naturally Wall-less Genus Mycloplasma
INCLUSIONS Within the cytoplasm of procaryotic(and eucaryotic) cells are several kinds of reserve deposits, known as inclusions. Some inclusions are common to a wide variety of bacteria. whereas others are limited to a small number of species and therefore serve as a basis for identification. Among the more prominent bacterial inclusions are the following Carbon storage polymers- PHB and glycogen Phosphate polymers Sulfur granules Gas Vacuoles
Within the cytoplasm of procaryotic (and eucaryotic) cells are several kinds of reserve deposits, known as inclusions. Some inclusions are common to a wide variety of bacteria, whereas others are limited to a small number of species and therefore serve as a basis for identification. Among the more prominent bacterial inclusions are the following: Carbon storage polymers – PHB and glycogen Phosphate polymers Sulfur Granules Gas Vacuoles INCLUSIONS
Gram Positive Gram Negative Fixation Crystal violet lodine treatment Decolorization Counter stain safranin
L-alanine D-glutamic acid L-lysine/Diaminopimelic acid D-alanine D-alanine Peptidoglycan ○○○ Muramic acid Glucosamine
Peptidoglycan Muramic acid Glucosamine L-alanine D-glutamic acid L-lysine/Diaminopimelic acid D-alanine D-alanine