第1篇有关人类RFLP实验论文 A Highly Polymorphic locus in Human DNA Arlene r. Wyman and ray white, MIT A locus in the human genome, not associated with any specific gene has been found to be a site of restriction fragment length polymorphism. The polymorphism was found by hybridizing a 16 kilobase-pair segment of single-copy human DNA, selected from the human genome library cloned in phage CH4A, to a Southern transfer of total human dna digested with EcoRI DNAs from a number of individuals from within mormon pedigrees as well as random individuals have been examined The locus is highl variable, with at least eight alleles present, homozygotes accounting for less than 25% of the individuals examined PNAS NoVember 1, 1980 vol 77 no 11 6754-6758
第1篇有关人类RFLP实验论文 A Highly Polymorphic Locus in Human DNA Arlene R. Wyman and Ray White, MIT A locus in the human genome, not associated with any specific gene, has been found to be a site of restriction fragment length polymorphism. The polymorphism was found by hybridizing a 16- kilobase-pair segment of single-copy human DNA, selected from the human genome library cloned in phage CH4A, to a Southern transfer of total human DNA digested with EcoRI. DNAs from a number of individuals from within Mormon pedigrees as well as random individuals have been examined. The locus is highly variable, with at least eight alleles present, homozygotes accounting for less than 25% of the individuals examined. ---PNAS | November 1, 1980 | vol. 77 | no. 11 | 6754-6758
最早涉及RFLP的论文 1)Botstein D, white Rl, skolnick M, Davis Rw(1980)Construction of a genetic lin kage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32: 314-331 PubMed 2. Gusella Jf, Wexler NS, Conneally PM, Naylor SL, Anderson MA Tanzi re, Watkins PC, ottina K, Wallace mr, sakaguchiAY Young AB, Shoulson I, Bonilla E, Martin JB(1983) Polymorphic DNA marker genetically linked to Huntingtons disease. Nature 306:234-238[ ubMed doi:10.1038/306234a0. 3. Knowlton RG, Cohen-Haguenauer O, van Cong n, Frezal J, Brown vA barker d, braman JC Schumm JW. tsui l inked to cystic fibrosis is located on chromosome 7. Natll arker Buchwald M, Donis-Keller H(1985 )A polymorphic DNA ma 318:380-382[ PubMed]doi:10.1038/318380a0
最早涉及RFLP的论文 1) Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331 [PubMed] 2. Gusella JF, Wexler NS, Conneally PM, Naylor SL, Anderson MA, Tanzi RE, Watkins PC, Ottina K, Wallace MR, Sakaguchi AY, Young AB, Shoulson I, Bonilla E, Martin JB (1983) Polymorphic DNA marker genetically linked to Huntington’s disease. Nature 306:234–238 [PubMed] doi: 10.1038/306234a0. 3. Knowlton RG, Cohen-Haguenauer O, Van Cong N, Frezal J, Brown VA, Barker D, Braman JC, Schumm JW, Tsui LC, Buchwald M, Donis-Keller H (1985) A polymorphic DNA marker linked to cystic fibrosis is located on chromosome 7. Nature 318:380–382 [PubMed] doi: 10.1038/318380a0
RFLP多态性的产生与检测(1) restriction site locations Hb A 1.15kb probe Hb S 135kb 口 135 1.15 AS AS NA S/S AS AS 子代两个亲本基因型,两个重组基因型
RFLP多态性的产生与检测(1) 子代两个亲本基因型, 两个重组基因型
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RFLP多态性的产生与检测(2)
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亲子 代 RFLP 多 态 性 的 检 测