Morphology Leptospira are tightly coiled and flexible(5-15 um long and 0.1-0.2 um wide). One or two ends are usually bent to form hook-like shape. wHile stained with silver stain(Fontana stain), they show a deep brown color
11 Morphology •Leptospira are tightly coiled and flexible (5-15 µ m long and 0.1-0.2 µ m wide). One or two ends are usually bent to form hook-like shape. •While stained with silver stain (Fontana stain), they show a deep brown color
Light Human Eye Electron Microscope Microscope Microscopi Application Units and I meter or symbols 1,000 mm 100 mm 10 mm(I mm/ 100 um 10 um I um/ 100 nm 10 nm(I nm Fraction of 10 101 10 10 107 9 a meter (m)
12
Morphology e They are actively mobile, which is best seen using a darkfield microscope
13 Morphology •They are actively mobile, which is best seen using a darkfield microscope
Ocular Lens Coarse Focus o Can magnify Fine Focus 1000 times Objective Lens stage Specimen Bulb
14 Can magnify 1000 times
Phase Contrast Light Pathways oobserve living cells Phase Ring Deflected Light Objective pecTen Condenser Annular Ring Light from Source
15 observe living cells