CHAPTER& The replication of nA The mechanism of DNA Polymerase (Pol) 3/18/05 Copyright 2004 Pearson Education, Inc, publishing as Benjamin Cummings @博共字 Wuhan Universit
The mechanism of DNA Polymerase (Pol) CHAPTER 8 The replication of DNA 3/18/05
DNA Pol use a single active site to catalyze DNA synthesis P A single site to catalyze the addition of any of the four dNTPs. Recognition of different dNTP by 2 monitoring the ability of incoming dNTP in forming A-T and G-c base pairs; incorrect base pair dramatically lowers the rate of catalysis( kinetic selectivity) 3/18/05
DNA Pol use a single active site to catalyze DNA synthesis A single site to catalyze the addition of any of the four dNTPs. Recognition of different dNTP by monitoring the ability of incoming dNTP in forming A-T and G-C base pairs; incorrect base pair dramatically lowers the rate of catalysis (kinetic selectivity). 3/18/05 The mechanism of DNA Pol
a correct base pair b incorrect base pair template C-0-P—0 C-0—P-0 no base G X dNTP dNTP OH OH primer 长 P=0 O=P-0 P Copyright O 2004 Pearson Education, Inc, publishing as Benge Figure 8-3
Figure 8-3
Distinguish between rNTP and dNTP by steric exclusion of rNTPs from the active site 030339 template c-0 discriminance dNP O P-0 DNA polymerase Fic gure 8-4 3/18/05
Distinguish between rNTP and dNTP by steric exclusion of rNTPs from the active site. 3/18/05 The mechanism of DNA Pol Figure 8-4
DNA Pol resemble a hand that grips the primer-template junction 030339 Schematic drawing T7 DNA pol u中 Fiqure 8-5
DNA Pol resemble a hand that grips the primer-template junction The mechanism of DNA Pol Figure 8-5 Schematic drawing T7 DNA pol