B D E FIG 8.1, In Vitro regeneration of plants through tissue culture. (A)Source of tissue explant (B) Explam culture on agar medium. (C) Callus production. (D) Plantct regenerated. (E Pander transplanted into stele soul
c E FIG. 8.3. Meristem-tip culture. (A)Apical meristem showing seeton to be excised. (B) Excised meristem tip cultured on agar medium. (C) Plantlet regenerated from excised meristem tip. (D) Plandet transferred to sterile sod. (E)Virus. free pbnd growing in soil
888 FG. 8.8. Somaclonal variation. (A) Haploid plant from which cultured tissue was obtined. (B) Cell suspension culture fmm haploid plant. (C) Mutant cell in suspension culnare. (D) Aggregate of mutant cells. (E) Haploid plandet that exhibits the mutant character. Culturing celts from haploid plants permits either dominant or recessive mutations to be exhibited in the regenerated plantlet. If tissue from diploid plant b culured, only dominant mutations will be Immediately expressed but recessive mutations will be expressed in some progeny plants as a result of segregation
FIG, 8. 4. Embryo culture. (A) Proembryo dissected 3 to 5 days after pollination. (B) Proembryo cured on solid agar medam (C) Plantlet developing from embryo. (D) Plandet tranplanted into soul
植物细胞培养( plant cell culture) ·植物细胞培养与微生物的区别: 细胞的大小 细胞块的形状 培养液的黏度 需氧量 K值比微生物培养的Km值小得多 对剪切力敏感 需CO2和光照
• 植物细胞培养与微生物的区别： 细胞的大小 细胞块的形状 培养液的黏度 需氧量 Kla 值比微生物培养的Kla值小得多 对剪切力敏感 需CO2和光照 植物细胞培养(plant cell culture)