实验八 细菌的鉴定（BBL半自 动鉴定系统） 一、教学要求 了解不同的微生物快速鉴定方法及应用 初步掌握细菌自动鉴定系统 （BBL CRYSTAL）的操作 技术 二、实验原理 1、 Miniaturized Biochemical Assays Bacteria can be identified by specific biochemical products they produce during growth. Biochemical and enzymatic reactions Miniaturized biochemical assays test for these specific products
实验八 细菌的鉴定（BBL半自 动鉴定系统） 一、教学要求 了解不同的微生物快速鉴定方法及应用 初步掌握细菌自动鉴定系统 （BBL CRYSTAL）的操作 技术 二、实验原理 1、 Miniaturized Biochemical Assays Bacteria can be identified by specific biochemical products they produce during growth. Biochemical and enzymatic reactions Miniaturized biochemical assays test for these specific products
API- Identification Kits Gram-negative bacteria Staphylococci
API- Identification Kits Gram-negative bacteria Staphylococci
2、Immunoassays ⚫ 血清学反应（Serological reactions） Agglutination reactions、Precipitation reactions ⚫ 免疫标记（immunolabel) Radioimmunoassays (RIA) Fluorescent immunoassays (FIA) Enzyme-linked immunosorbent assays (ELISA) Chemiluminesent Immunoassay(CLIA)、 Time-resolved fluorescent immunoassays（TRFIA） Immunogold labelling
2、Immunoassays ⚫ 血清学反应（Serological reactions） Agglutination reactions、Precipitation reactions ⚫ 免疫标记（immunolabel) Radioimmunoassays (RIA) Fluorescent immunoassays (FIA) Enzyme-linked immunosorbent assays (ELISA) Chemiluminesent Immunoassay(CLIA)、 Time-resolved fluorescent immunoassays（TRFIA） Immunogold labelling
ELISA（enzyme linked immunosorbent assay） The ELISA can be used to detect the presence of antigens that are recognized by an antibody, or it can be used to test for antibodies that recognize an antigen. ⚫ Sandwich ELISA
ELISA（enzyme linked immunosorbent assay） The ELISA can be used to detect the presence of antigens that are recognized by an antibody, or it can be used to test for antibodies that recognize an antigen. ⚫ Sandwich ELISA
Immunogold labelling techiques ⚫ 斑点免疫金渗滤法 ⚫ 胶体金标记免疫层析法
Immunogold labelling techiques ⚫ 斑点免疫金渗滤法 ⚫ 胶体金标记免疫层析法