TeachingPlanforBachelor of Medicine and Bachelor of Surgery (MBSS)Department of Histology, School of Medicine, Yangzou UniversityCourseHistology&EmbryologyTextbookTheTextbook of Histologyand Embryology (Yingmao Gao)Teaching TypeLectureRealia(1) PPT(2)OnlinePlatform(http://zzptx.yzuyxy.cn/en/)Reference BooksI. Junqueira's Basic Histology: Text and Atlas, 13e (Anthony Mescher)2. Langman's Medical Embryology (Thomas W. Sadler)Unit1 Introduction to HistologyClass Hours2Objectives1.TOMASTER(1) The definition of Histology;(2) The definition of Tissue and Four Basic Tissues;(3) Hematoxylin and Eosin (H.E) staining method;(4)The definition of basophilia, acidophilia, argentaffin and argyrophilia(5) The definition of ultrastructure;(6)PAS Reaction
Teaching Plan for Bachelor of Medicine and Bachelor of Surgery (MBSS) Department of Histology, School of Medicine, Yangzou University Course Histology & Embryology Textbook The Textbook of Histology and Embryology (Yingmao Gao) Teaching Type Lecture Realia （1）PPT （2）Online Platform（http://zzptx.yzuyxy.cn/en/） Reference Books 1. Junqueira's Basic Histology: Text and Atlas, 13e (Anthony Mescher) 2. Langman's Medical Embryology (Thomas W. Sadler) Unit 1 Introduction to Histology Class Hours 2 Objectives 1. TO MASTER (1) The definition of Histology; (2) The definition of Tissue and Four Basic Tissues; (3) Hematoxylin and Eosin (H.E) staining method; (4) The definition of basophilia, acidophilia, argentaffin and argyrophilia; (5) The definition of ultrastructure; (6) PAS Reaction
2.TOUNDERSTAND(1) The procedure of Paraffin Sectioning;(2) The difference between Transmission EM and Scanning EM;(3)Immunohistochemistry,in situhybridizationandcell cultureKeyPoints1.The definition of Tissue and Four Basic Tissues:2. Hematoxylin and Eosin (H.E) staining method;3.The definition of basophilia,acidophilia, argentaffin and argyrophilia;4. The difference between Transmission EM and Scanning EM;5.PAS ReactionDifficult Points1.HematoxylinandEosin (H.E)stainingmethod2. The definition of basophilia and acidophilia,3. The difference between Transmission EM and Scanning EM;4.PAS Reaction.Teaching contents (90 min)1.Definition and contents of Histology (10 min)2.Relationship of Histology to other medical courses (10 min)3. Studying method (70 min)a.Lightmicroscopy:Paraffin Sectioning andH&E staining(25min)b.Electron microscopy (20 min)c. Histochemistry and cytochemistry: PAS reaction (10 min)d. Immunocytochemistry (5 min)e. In situ hybridization (5 min)f. Cell culture (5 min)AssignmentAnswering Questions1. What is the H&E staining?2.What are the differences between the light and electron microscopy?3.Howdoesahistologist studythe livingcellsandtissues?
2. TO UNDERSTAND (1) The procedure of Paraffin Sectioning; (2) The difference between Transmission EM and Scanning EM; (3) Immunohistochemistry, in situ hybridization and cell culture. Key Points 1. The definition of Tissue and Four Basic Tissues; 2. Hematoxylin and Eosin (H.E) staining method; 3. The definition of basophilia, acidophilia, argentaffin and argyrophilia; 4. The difference between Transmission EM and Scanning EM; 5. PAS Reaction. Difficult Points 1. Hematoxylin and Eosin (H.E) staining method; 2. The definition of basophilia and acidophilia; 3. The difference between Transmission EM and Scanning EM; 4. PAS Reaction. Teaching contents (90 min) 1. Definition and contents of Histology (10 min) 2. Relationship of Histology to other medical courses (10 min) 3. Studying method (70 min) a. Light microscopy: Paraffin Sectioning and H&E staining (25 min) b. Electron microscopy (20 min) c. Histochemistry and cytochemistry: PAS reaction (10 min) d. Immunocytochemistry (5 min) e. In situ hybridization (5 min) f. Cell culture (5 min) Assignment Answering Questions: 1. What is the H&E staining? 2.What are the differences between the light and electron microscopy? 3. How does a histologist study the living cells and tissues?
Unit2EpithelialTissueClassHours4Objectives1.TOMASTER(1) The structural characteristics, distribution and function of the epithelium(2) The structure,function and distribution of simple squamous epithelium(including endothelium and mesothelium, simple cuboidal epithelium, simplecolumnar epithelium, pseudostratified columnar ciliated epithelium, stratifiedsquamous epithelium and transitional epithelium);(3)Thespecializations oftheepithelium:thespecializationsinfree surface(ciliaand microvilli), lateral surface (junctional complex)and basal surface (basementmembrane),(4) Concept of the glandular epithelium and gland;(5) Structural characteristics of the endocrine gland and exocrine gland2.TOUNDERSTAND(l) intercellular junctions (tight junction, desmosome, intermediate junction andgap junction);(2) The regeneration of the epithelium.KeyPoints1. The structural characteristics, distribution and function of the epithelium;2. The structure, function and distribution of simple squamous epithelium(includingendotheliumandmesothelium,simplecuboidalepithelium,simplecolumnar epithelium, pseudostratified columnar ciliated epithelium, stratifiedsquamous epithelium and transitional epithelium);3.The specializations of the epithelium:the specializations in free surface (ciliaand microvilli), lateral surface (junctional complex) and basal surface (basementmembrane);4. Concept of the glandular epithelium and gland;
Unit 2 Epithelial Tissue Class Hours 4 Objectives 1. TO MASTER (1) The structural characteristics, distribution and function of the epithelium; (2) The structure, function and distribution of simple squamous epithelium (including endothelium and mesothelium, simple cuboidal epithelium, simple columnar epithelium, pseudostratified columnar ciliated epithelium, stratified squamous epithelium and transitional epithelium); (3) The specializations of the epithelium: the specializations in free surface (cilia and microvilli), lateral surface (junctional complex) and basal surface (basement membrane); (4) Concept of the glandular epithelium and gland; (5) Structural characteristics of the endocrine gland and exocrine gland. 2. TO UNDERSTAND (1) intercellular junctions (tight junction, desmosome, intermediate junction and gap junction); (2) The regeneration of the epithelium. Key Points 1. The structural characteristics, distribution and function of the epithelium; 2. The structure, function and distribution of simple squamous epithelium (including endothelium and mesothelium, simple cuboidal epithelium, simple columnar epithelium, pseudostratified columnar ciliated epithelium, stratified squamous epithelium and transitional epithelium); 3. The specializations of the epithelium: the specializations in free surface (cilia and microvilli), lateral surface (junctional complex) and basal surface (basement membrane); 4. Concept of the glandular epithelium and gland;
5. Structural characteristics of the endocrine gland and exocrine glandDifficultPoints1. The structure, function and distribution of pseudostratified columnar ciliatedepithelium and transitional epithelium;2. Cilia and microvilli;3.Tight junction, desmosome, intermediate junction and gap junction;4. Structural characteristics of Serous gland cell and mucous gland cellTeaching contents (180 min)1. Classification and general features of the epithelial tissues (20 min)2. Covering epithelia: type, distribution, and function (50 min)3.Epithelial specializations (60 min)a. Free surface: microvilli, cilia (15 min)b.Lateral surface: cell junctions (25 min)c.Basal surface: basement membrane, plasma membrane infolding,hemidesmosome(20 min)4. Glandular epithelia and glands (40 min)5.Renewal of epithelial tissue (10min)AssignmentExplanation of Terms1.Microvilli2.Cilia3. Gap junction4.Basement membraneAnswering Questions1.Explainthe structural characteristics andbasis of classification of epithelium2.Comparemicrovillus with ciliumUnit3ConnectiveTissueClass Hours3
5. Structural characteristics of the endocrine gland and exocrine gland. Difficult Points 1. The structure, function and distribution of pseudostratified columnar ciliated epithelium and transitional epithelium; 2. Cilia and microvilli; 3. Tight junction, desmosome, intermediate junction and gap junction; 4. Structural characteristics of Serous gland cell and mucous gland cell. Teaching contents (180 min) 1. Classification and general features of the epithelial tissues (20 min) 2. Covering epithelia: type, distribution, and function (50 min) 3. Epithelial specializations (60 min) a. Free surface: microvilli, cilia (15 min) b. Lateral surface: cell junctions (25 min) c. Basal surface: basement membrane, plasma membrane infolding, hemidesmosome (20 min) 4. Glandular epithelia and glands (40 min) 5. Renewal of epithelial tissue (10 min) Assignment Explanation of Terms 1.Microvilli 2. Cilia 3. Gap junction 4. Basement membrane Answering Questions 1. Explain the structural characteristics and basis of classification of epithelium. 2. Compare microvillus with cilium. Unit 3 Connective Tissue Class Hours 3
Objectives1.TOMASTER(1) The structural characteristics and classification of the connective tissue;(2) The structural characteristic and function of loose connective tissue;(3) The chemical constituents, morphological structure and function of 3 fiber types(collagenous, elastic and reticular fibers),(4) The chemical constituents and characteristics of the matrix of intercellularsubstance;(5) The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell)2.TOUNDERSTAND(1) The structural characteristics and function of dense connective tissue, adiposetissue, reticular tissue and the tissue fluid.KeyPoints1. The structural characteristics and classification of the connective tissue;2. The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell),3.The chemical constituents, morphological structure and function of three fibertypes (collagenous, elastic and reticular fibers);4. The chemical constituents and characteristics of the matrix of intercellularsubstanceDifficult Points1.The structure and function of several main cell types in connective tissue(fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell):2. The chemical constituents, morphological structure and function of three fibertypes (collagenous, elastic and reticularfibers);3. The chemical constituents and characteristics of the matrix of intercellularsubstanceTeaching contents (135 min)1. Classification and general characteristics of connective tissue (20 min)
Objectives 1. TO MASTER (1) The structural characteristics and classification of the connective tissue; (2) The structural characteristic and function of loose connective tissue; (3) The chemical constituents, morphological structure and function of 3 fiber types (collagenous, elastic and reticular fibers); (4) The chemical constituents and characteristics of the matrix of intercellular substance; (5) The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell). 2. TO UNDERSTAND (1) The structural characteristics and function of dense connective tissue, adipose tissue, reticular tissue and the tissue fluid. Key Points 1. The structural characteristics and classification of the connective tissue; 2. The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell); 3. The chemical constituents, morphological structure and function of three fiber types (collagenous, elastic and reticular fibers); 4. The chemical constituents and characteristics of the matrix of intercellular substance. Difficult Points 1. The structure and function of several main cell types in connective tissue (fibroblast, macrophage, plasma cell, mast cell, fat cell and mesenchymal cell); 2. The chemical constituents, morphological structure and function of three fiber types (collagenous, elastic and reticular fibers); 3. The chemical constituents and characteristics of the matrix of intercellular substance. Teaching contents (135 min) 1. Classification and general characteristics of connective tissue (20 min)