⑧ 有效放大倍率 Resolution of naked eye Effective magnification= Resolution of microscope Effective magnification (Mn: resolution of eye 0.2mm ≈1333× max.resolution of LOM 0.00015mm M's >Mr make the image bigger,but do not provide any additional details.This is termed "empty magnification. 材料科学与工程学院 6 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 有效放大倍率 6
@ Empty magnification Every magnification value that does not increase the amount of information already contained in an image obtained at some lower magnification,regardless of the amount of trouble that has to be expanded in the act of perceiving.-Van Dujin 材料科学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering Empty magnification • Every magnification value that does not increase the amount of information already contained in an image obtained at some lower magnification, regardless of the amount of trouble that has to be expanded in the act of perceiving. ——Van Dujin 7
从透镜到显微镜 A Some Types of Optical Microscopes 1.Simple optical microscope->One lens;25x;10 um resolution 2.Stereoscopic microscope →Two lens trains;6-8x 3.Compound optical microscope - Objective eyepiece condenser lenses;1300x;1 um resolution. 2 3 材料科学与工程学院 8 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 从透镜到显微镜 8
图 显微镜系统 物镜 Reflected Light Binocular Lamp House Head Aperture ·光学系统 Diaphragm Field Collector Eyepieces Diaphragm Lens 照明系统 Vertical llluminator Filter Slider ·记录系统 Objective. Internal Mechanical Circuit 载物台 Board Stage Condenser ·控制系统 Base Field Diaphragm Filters Transmitted Light Lamp House 材料科学与工程学院 9 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 显微镜系统 • 物镜 • 光学系统 • 照明系统 • 记录系统 • 载物台 • 控制系统 9
显微镜的镜头/物镜 Image formation in a reflected light microscope lllumination Similar to transmission source optical microscope. Different arrangement of components Condenser aperture and lens Half-silvered (dichromatic) mirror B Object 放大倍数 Final (specimen) First 数值孔径 Image Image Objective 分辨率 aperture and lens Depth of field 像差校正 物镜 物镜类型 材科科学与工程学院 10 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 显微镜的镜头/物镜 10 • 放大倍数 • 数值孔径 • 分辨率 • Depth of field • 像差校正 物镜 • 物镜类型