Replication of CoE1 plasmid DNA-multicopy control -555 265 -20 P origIn 3 P RNA II serves as primer for DNa synthesis for RNA‖l replication. 5 Replicase
P-II origin Replication of ColE1 plasmid DNA-multicopy control RNA II -555 -20 5’ 3’ RNA II serves as primer for DNA synthesis for replication. P-I Replicase -265
Replication of ColE1 plasmid DNA-rnulticopy control -555 20 P origIn 3 RNA I No replication but 5 RNa l transcription continues Without loops RNA Il is not able DNA synthesis can not to form a proceed without a persistent hybrid at stable primer the origin
P-II origin Replication of ColE1 plasmid DNA-multicopy control RNA II -555 -20 Without loops, RNA II is not able to form a persistent hybrid at the origin. P-I RNA I DNA synthesis can not proceed without a stable primer 3’ No replication but transcription continues 5’
质粒拷贝数自主调控 RNA I initiation of transcription proceeds as much as five times more often than transcription of rNa II. as a consequence only one out of about 20 RNA I molecules that are initiated results in a productive DNA replication Rom蛋白可与RNAI的茎干结合,使其稳定 可提高复制起始.也可与RNA和 RNAII 双链结合,降低RNAI的起始效率
质粒拷贝数自主调控 RNA I initiation of transcription proceeds as much as five times more often than transcription of RNA II. As a consequence only one out of about 20 RNA II molecules that are initiated results in a productive DNA replication. Rom蛋白可与RNAII的茎干结合, 使其稳定, 可提高复制起始. 也可与RNAI和RNAII 双链结合, 降低RNAII的起始效率
F质粒单拷贝复制调控 RepE monomer Initiate repE mRNA replication卜 DnaA AT 13mer DR IR (P/O repE incc SopA, B x■ 【【■ mRNA Repress transcription RepE dimer Dnaa box:DnaA蛋白结合区,A/T:富AT区.13mer:OrC区13bp同源序 列DR:顺式重复顺序,与repE蛋白结合,使双链DNA分开IR:反向重复 顺序,顺序与DR一致.incC:拷贝数控制区.ori2:质粒复制起始点 spA4,BC:参与质粒分配到子细胞PO:启动子/操纵基因 repE为复制蛋白,单体时可与DR重复顺序结合起始复制repE在二聚体 时与I结合,抑制复制起始和转录
F质粒单拷贝复制调控 DnaA box: DnaA蛋白结合区. A/T:富A/T区. 13mer:OriC区13bp同源序 列. DR: 顺式重复顺序, 与repE蛋白结合, 使双链DNA分开. IR: 反向重复 顺序, 顺序与DR一致. incC:拷贝数控制区. ori2: 质粒复制起始点. sopA,B,C: 参与质粒分配到子细胞. P/O: 启动子/操纵基因. repE为复制蛋白, 单体时可与DR重复顺序结合起始复制. repE在二聚体 时与IR结合, 抑制复制起始和转录